Liu Y, Zhou Y L, Qian Y Y, Wang Y J, Ding F, Gu X S, Liu M
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, PR China.
Genet Mol Res. 2010 Aug 17;9(3):1606-14. doi: 10.4238/vol9-3gmr888.
The cDNA encoding noggin2 protein was obtained from the brain and spinal cord cDNA library of Gekko japonicus. The size of the noggin2 transcript and its expression in different tissues were analyzed by Northern blot analysis. In situ hybridization revealed positive hybridization signals in both gray and white matter of the spinal cord. Changes in noggin2 expression in the spinal cord after tail amputation were examined by real-time PCR. The noggin2 was expressed in the normal spinal cord and down-regulated three days after tail amputation, reaching the lowest level at two weeks, during the time course when we followed the expression levels. We concluded that the expression of noggin2 is affected by the process of spinal cord injury and regeneration.
从日本壁虎的脑和脊髓cDNA文库中获得了编码头蛋白2（noggin2）的cDNA。通过Northern印迹分析对头蛋白2转录本的大小及其在不同组织中的表达进行了分析。原位杂交显示脊髓灰质和白质均有阳性杂交信号。通过实时PCR检测了断尾后脊髓中头蛋白2表达的变化。在我们跟踪表达水平的时间进程中，头蛋白2在正常脊髓中表达，断尾后三天表达下调，在两周时达到最低水平。我们得出结论，头蛋白2的表达受脊髓损伤和再生过程的影响。
