保幼激素对冈比亚按蚊雌性生殖的作用方式。
Mode of action of methoprene in affecting female reproduction in the African malaria mosquito, Anopheles gambiae.
机构信息
Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.
出版信息
Pest Manag Sci. 2010 Sep;66(9):936-43. doi: 10.1002/ps.1962.
BACKGROUND
One of the most studied actions of juvenile hormone (JH) is its ability to modulate ecdysteroid signaling during insect development and metamorphosis. Previous studies in mosquitoes showed that 20-hydroxyecdysone (20E) regulates vitellogenin synthesis. However, the action of JH and its mimics, e.g. methoprene, on female reproduction of mosquitoes remains unknown.
RESULTS
Here, a major malaria vector, Anopheles gambiae Giles, was used as a model insect to study the action of methoprene on female reproduction. Ecdysteroid titers and expression profiles of ecdysone-regulated genes were determined before and after a blood meal. An ecdysteroid peak was detected at 12 h post blood meal (PBM). The maximum expression of ecdysone-regulated genes, such as ecdysone receptor (EcR), hormone receptor 3 (HR3) and vitellogenin (Vg) gene, coincided with the ecdysteroid peak. Interestingly, topical application of methoprene at 6 h PBM delayed ovarian development and egg maturation by suppressing the expression of ecdysone-regulated genes in female mosquitoes.
CONCLUSION
The data suggest that ecdysteroid titers are correlated with Vg synthesis, and methoprene affects vitellogenesis by modulating ecdysteroid action in A. gambiae.
背景
研究最多的保幼激素（JH）作用之一是其在昆虫发育和变态过程中调节蜕皮激素信号的能力。先前在蚊子中的研究表明，20-羟基蜕皮酮（20E）调节卵黄蛋白原的合成。然而，JH 及其类似物，例如灭幼脲，对蚊子的雌性生殖的作用仍然未知。
结果
在这里，以主要的疟疾媒介，冈比亚按蚊 Giles，作为模型昆虫来研究灭幼脲对雌性生殖的作用。在吸血前后测定蜕皮激素滴度和蜕皮激素调节基因的表达谱。在吸血后 12 小时（PBM）检测到蜕皮激素峰。蜕皮激素调节基因，如蜕皮激素受体（EcR）、激素受体 3（HR3）和卵黄蛋白原（Vg）基因的最大表达与蜕皮激素峰同时发生。有趣的是，在 6 h PBM 时局部施用灭幼脲通过抑制雌性蚊子中蜕皮激素调节基因的表达来延迟卵巢发育和卵成熟。
结论
数据表明，蜕皮激素滴度与 Vg 合成相关，灭幼脲通过调节 A. gambiae 中的蜕皮激素作用来影响卵黄发生。
Zotero 插件