保幼激素对冈比亚按蚊雌性生殖的作用方式。
Mode of action of methoprene in affecting female reproduction in the African malaria mosquito, Anopheles gambiae.
机构信息
Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.
出版信息
Pest Manag Sci. 2010 Sep;66(9):936-43. doi: 10.1002/ps.1962.
Abstract
BACKGROUND
One of the most studied actions of juvenile hormone (JH) is its ability to modulate ecdysteroid signaling during insect development and metamorphosis. Previous studies in mosquitoes showed that 20-hydroxyecdysone (20E) regulates vitellogenin synthesis. However, the action of JH and its mimics, e.g. methoprene, on female reproduction of mosquitoes remains unknown.
RESULTS
Here, a major malaria vector, Anopheles gambiae Giles, was used as a model insect to study the action of methoprene on female reproduction. Ecdysteroid titers and expression profiles of ecdysone-regulated genes were determined before and after a blood meal. An ecdysteroid peak was detected at 12 h post blood meal (PBM). The maximum expression of ecdysone-regulated genes, such as ecdysone receptor (EcR), hormone receptor 3 (HR3) and vitellogenin (Vg) gene, coincided with the ecdysteroid peak. Interestingly, topical application of methoprene at 6 h PBM delayed ovarian development and egg maturation by suppressing the expression of ecdysone-regulated genes in female mosquitoes.
CONCLUSION
The data suggest that ecdysteroid titers are correlated with Vg synthesis, and methoprene affects vitellogenesis by modulating ecdysteroid action in A. gambiae.
摘要
背景
研究最多的保幼激素（JH）作用之一是其在昆虫发育和变态过程中调节蜕皮激素信号的能力。先前在蚊子中的研究表明，20-羟基蜕皮酮（20E）调节卵黄蛋白原的合成。然而，JH 及其类似物，例如灭幼脲，对蚊子的雌性生殖的作用仍然未知。
结果
在这里，以主要的疟疾媒介，冈比亚按蚊 Giles，作为模型昆虫来研究灭幼脲对雌性生殖的作用。在吸血前后测定蜕皮激素滴度和蜕皮激素调节基因的表达谱。在吸血后 12 小时（PBM）检测到蜕皮激素峰。蜕皮激素调节基因，如蜕皮激素受体（EcR）、激素受体 3（HR3）和卵黄蛋白原（Vg）基因的最大表达与蜕皮激素峰同时发生。有趣的是，在 6 h PBM 时局部施用灭幼脲通过抑制雌性蚊子中蜕皮激素调节基因的表达来延迟卵巢发育和卵成熟。
结论
数据表明，蜕皮激素滴度与 Vg 合成相关，灭幼脲通过调节 A. gambiae 中的蜕皮激素作用来影响卵黄发生。
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本文引用的文献
1
Juvenile hormone regulation of male accessory gland activity in the red flour beetle, Tribolium castaneum.赤拟谷盗（Tribolium castaneum）中保幼激素对雄性附腺活性的调节
Mech Dev. 2009 Jul;126(7):563-79. doi: 10.1016/j.mod.2009.03.005. Epub 2009 Mar 24.
2
Target of rapamycin (TOR) mediates the transduction of nutritional signals into juvenile hormone production.雷帕霉素靶蛋白（TOR）介导营养信号向保幼激素产生的转导。
J Biol Chem. 2009 Feb 27;284(9):5506-13. doi: 10.1074/jbc.M807042200. Epub 2008 Dec 23.
3
Molecular analysis of juvenile hormone analog action in controlling the metamorphosis of the red flour beetle, Tribolium castaneum.保幼激素类似物调控赤拟谷盗（Tribolium castaneum）变态发育的分子分析
Arch Insect Biochem Physiol. 2009 Jan;70(1):57-70. doi: 10.1002/arch.20288.
4
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Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19631-6. doi: 10.1073/pnas.0809264105. Epub 2008 Dec 5.
5
Transcription factor broad suppresses precocious development of adult structures during larval-pupal metamorphosis in the red flour beetle, Tribolium castaneum.转录因子broad抑制赤拟谷盗在幼虫-蛹变态过程中成虫结构的过早发育。
Mech Dev. 2008 Mar-Apr;125(3-4):299-313. doi: 10.1016/j.mod.2007.11.001. Epub 2007 Nov 17.
6
Induction of follicle separation in the mosquito by physiological amounts of ecdysterone.用生理剂量的蜕皮甾酮诱导蚊子卵泡分离。
Science. 1980 Aug 15;209(4458):819-21. doi: 10.1126/science.209.4458.819.
7
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Mech Dev. 2007 Jan;124(1):23-34. doi: 10.1016/j.mod.2006.09.002. Epub 2006 Sep 23.
8
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Mech Dev. 2006 Jul;123(7):530-47. doi: 10.1016/j.mod.2006.05.005. Epub 2006 May 25.
9
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J Insect Physiol. 2006 Jun;52(6):558-68. doi: 10.1016/j.jinsphys.2006.02.003. Epub 2006 Mar 31.
10
A standard curve based method for relative real time PCR data processing.一种基于标准曲线的相对实时PCR数据处理方法。
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