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保幼激素和蜕皮甾类通过雌性体内的[基因名称]和[基因名称]促进成虫繁殖。 （原文中两个“Gene”处应是具体基因名称，但未给出，所以只能这样翻译）

Juvenile Hormone and Ecdysteroids Facilitate the Adult Reproduction Through the Gene and Gene in the Female .

作者信息

Zhang Yan, Ding Kui-Ting, Yang Nian-Wan, Lv Zhi-Chuang, Wang Zhen-Ying, Zhang Yong-Jun, Liu Wan-Xue, Guo Jian-Yang

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

出版信息

Int J Mol Sci. 2025 Feb 23;26(5):1914. doi: 10.3390/ijms26051914.

DOI:10.3390/ijms26051914
PMID:40076541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11900537/
Abstract

Insects, as the most diverse and numerous group in the animal kingdom, are at least partly dependent on the reproduction process, which is strictly regulated by the 'classic' insect hormones: juvenile hormone (JH), and 20-hydroxyecdysone (20E). However, the regulatory mechanism governing the reproduction of JH and 20E in remains unclear. In this study, ovarian development and ovulation in female were assessed through dissection of the ovaries following treatment with JH analog (JHA) and 20E. Moreover, the expression patterns of the JH-signal and 20E-signal-related genes were determined by quantitative PCR (qPCR), and RNA interference (RNAi) was used to investigate the role of JH and 20E-induced genes. Ovarian development was observed by microdissection, and JH and 20E titers were determined by ELISA. , , and expression were determined by qPCR. Dissection and qPCR results showed that JHA and 20E promoted ovarian development, egg maturation, and egg laying by upregulating () and ()expression. Additionally, the RNAi results showed that the injection of ds and ds markedly delayed ovarian development, inhibited egg maturation, and halted egg production. Knockdown of and significantly reduced JH and 20E content and inhibited the transcription of and . These results indicate that JH and 20E facilitate adult reproduction through the methoprene-tolerant gene and ecdysone receptor gene in female

摘要

昆虫作为动物界中种类最多、数量最庞大的类群，至少部分依赖于繁殖过程，而繁殖过程受到 “经典” 昆虫激素的严格调控：保幼激素（JH）和 20 - 羟基蜕皮酮（20E）。然而，JH 和 20E 在[具体昆虫名称未给出]中调控繁殖的机制仍不清楚。在本研究中，通过对用 JH 类似物（JHA）和 20E 处理后的雌性[具体昆虫名称未给出]进行卵巢解剖，评估其卵巢发育和排卵情况。此外，通过定量 PCR（qPCR）确定 JH 信号和 20E 信号相关基因的表达模式，并利用 RNA 干扰（RNAi）研究 JH 和 20E 诱导基因的作用。通过显微解剖观察卵巢发育情况，并用 ELISA 测定 JH 和 20E 的滴度。通过 qPCR 测定[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的表达。解剖和 qPCR 结果表明，JHA 和 20E 通过上调[具体基因名称未给出]（[基因全名未给出]）和[具体基因名称未给出]（[基因全名未给出]）的表达来促进卵巢发育、卵成熟和产卵。此外，RNAi 结果表明，注射双链 RNA（ds）[具体基因名称未给出]和 ds[具体基因名称未给出]显著延迟了卵巢发育，抑制了卵成熟，并停止了产卵。敲低[具体基因名称未给出]和[具体基因名称未给出]显著降低了 JH 和 20E 的含量，并抑制了[具体基因名称未给出]和[具体基因名称未给出]的转录。这些结果表明，JH 和 20E 通过雌性[具体昆虫名称未给出]中的抗蚊蝇醚基因和蜕皮激素受体基因促进成虫繁殖

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fe3/11900537/245cfefc2f79/ijms-26-01914-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fe3/11900537/bde1533b4467/ijms-26-01914-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fe3/11900537/e3f9cf555b21/ijms-26-01914-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fe3/11900537/dec04baa6fac/ijms-26-01914-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fe3/11900537/590a799c667c/ijms-26-01914-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fe3/11900537/0dee1c159178/ijms-26-01914-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fe3/11900537/245cfefc2f79/ijms-26-01914-g008.jpg

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