Nemoto K, Okamura T
Department of Pharmacology, Nippon Medical School.
Arerugi. 1990 Jul;39(7):595-604.
Intracellular signal transduction during anaphylactic contractions of smooth muscle and the site of signal generation, or antigen-antibody reaction, participating in the appearance of these contractions were studied. The taenia coli was removed from a guinea-pig which had previously been sensitized with anti-egg albumin (EA) rabbit serum or anti-EA rabbit immunoglobulin G (IgG) fraction. Dispersed smooth muscle cells, obtained after enzymatic digestion, were used as the specimen. The dispersed single smooth muscle cells sensitized with anti-EA rabbit IgG fraction showed anaphylactic contractions in response to antigen administration. The method using enzyme-linked antibody revealed binding of IgG by these single muscle cells. Anaphylactic contractions of the single cells were not inhibited by antihistamines or anti-5-hydroxy-tryptamine (5HT) agents, but they were suppressed by Y-19,432 [1-butyl-5-hydroxy-2-methyl-N-[1-(2-phenylethyl)-4-piperidyl] indole-3-carboxamide hydrochloride hydrate], an inhibitor of 5-lipoxygenase. The anaphylactic contractions of the single smooth muscle cells of the guinea-pig taenia coli appear to be initiated in response to IgG binding with the antigen bound to the smooth muscle plasma membrane. For contraction development, the lipoxygenase pathway was shown to participate as a part of the intracellular signal transduction.
研究了平滑肌过敏反应性收缩过程中的细胞内信号转导以及参与这些收缩出现的信号产生部位，即抗原 - 抗体反应。从先前用抗卵清蛋白（EA）兔血清或抗EA兔免疫球蛋白G（IgG）组分致敏的豚鼠中取出结肠带。将酶消化后获得的分散平滑肌细胞用作标本。用抗EA兔IgG组分致敏的分散单个平滑肌细胞在给予抗原后表现出过敏反应性收缩。使用酶联抗体的方法显示这些单个肌肉细胞结合了IgG。单细胞的过敏反应性收缩不受组胺拮抗剂或抗5 - 羟色胺（5HT）剂的抑制，但被5 - 脂氧合酶抑制剂Y - 19,432 [1 - 丁基 - 5 - 羟基 - 2 - 甲基 - N - [1 - （2 - 苯乙基） - 4 - 哌啶基]吲哚 - 3 - 甲酰胺盐酸盐水合物]抑制。豚鼠结肠带单个平滑肌细胞的过敏反应性收缩似乎是在IgG与结合于平滑肌质膜的抗原结合后启动的。对于收缩的发展，脂氧合酶途径被证明作为细胞内信号转导的一部分参与其中。
