Husøy T, Syversen T, Jenssen J
University of Trondheim, Faculty of Medicine, Department of Pharmacology and Toxicology, Medisinsk Teknisk Senter, N-7005 Trondheim, Norway.
Toxicol In Vitro. 1993 Mar;7(2):149-54. doi: 10.1016/0887-2333(93)90125-o.
Four different in vitro cytotoxicity tests were compared: the MTT assay, the NR assay, the uridine incorporation assay and the measurement of total cellular protein. The comparison was done using the BHK-21 cell line and nine selected test chemicals (colchicine, amitriptyline, cycloheximide, 2,5-hexandione, mercury chloride, cadmium chloride, copper chloride, 2,4-dinitrophenol and chloroquine diphosphate). The concentration that induced 50% inhibition relative to the controls (IC(50)) was calculated for each test and chemical. The results from the cytotoxicity tests were generally in good agreement. However, for some chemicals the IC(50) values varied significantly between two assays. The largest variation was found for chloroquine diphosphate, where the IC(50) value for the NR assay was approximately eight times lower than the IC(50) value for the protein measurement.
MTT 法、NR 法、尿苷掺入法和总细胞蛋白测量法。使用 BHK - 21 细胞系和九种选定的测试化学品（秋水仙碱、阿米替林、环己酰亚胺、2,5 - 己二酮、氯化汞、氯化镉、氯化铜、2,4 - 二硝基苯酚和二磷酸氯喹）进行了比较。计算每种试验和化学品相对于对照诱导 50%抑制的浓度（IC(50)）。细胞毒性试验的结果总体上吻合良好。然而，对于某些化学品，两种试验之间的 IC(50)值差异显著。二磷酸氯喹的差异最大，其中 NR 试验的 IC(50)值比蛋白质测量的 IC(50)值低约八倍。
