Abir R, Ornoy A, Ben Hur H, Jaffe P, Pinus H
Laboratory of Teratology, Department of Anatomy and Embryology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Toxicol In Vitro. 1993 Nov;7(6):817-26. doi: 10.1016/0887-2333(93)90086-k.
Many recurrent abortions have an unknown aetiology. A significant proportion of sera from women with spontaneous abortions induced 50% or more anomalies ('high risk' sera) in cultures of 10.5-day-old rat embryos. Because it is assumed that the teratogenic factor(s) in recurrent abortion is often an immunoglobulin G (IgG) antibody, it was attempted to reduce the rate of anomalies induced by high risk sera by removing the IgG fractions and adding IgG fractions from control sera (sera from women in a normal second trimester of pregnancy and after delivery). Concomitantly, an attempt was made to induce anomalies in embryos cultured in control sera by adding IgG fractions from high risk sera. It was found that among the high risk sera there were three groups, according to the response to the IgG exchange: (1) a group in which IgG exchange 'corrected' the anomalies in high risk sera, whereas it induced anomalies in control sera; (2) a group in which anomalies were found both in embryos cultured in high risk sera and those cultured in control sera; (3) a group in which the IgG exchange did not affect the results in the 'experimental' or 'control' embryos. Examination by transmission electron microscopy, of yolk sacs cultured in sera with IgG from women belonging to the third group did not reveal ultrastructural changes. In conclusion, in some high risk sera the teratogenic factor appears to be an IgG only; in others, teratogenicity is brought about not only by an IgG, but also by additional teratogenic factor(s); in the last group, the teratogenic factor(s) does not appear to be an IgG at all.
许多复发性流产的病因不明。在10.5日龄大鼠胚胎培养中，很大一部分自然流产女性的血清会诱导出50%或更多的异常（“高危”血清）。由于假定复发性流产中的致畸因素通常是免疫球蛋白G（IgG）抗体，因此尝试通过去除IgG组分并添加对照血清（来自妊娠中期正常及产后女性的血清）的IgG组分来降低高危血清诱导的异常率。同时，尝试通过添加高危血清的IgG组分在对照血清中培养的胚胎中诱导异常。根据对IgG交换的反应，发现高危血清中有三组：（1）一组中，IgG交换“纠正”了高危血清中的异常，而在对照血清中诱导出异常；（2）一组中，在高危血清中培养的胚胎和对照血清中培养的胚胎均发现异常；（3）一组中，IgG交换对“实验性”或“对照”胚胎的结果没有影响。对第三组女性IgG血清中培养的卵黄囊进行透射电子显微镜检查，未发现超微结构变化。总之，在一些高危血清中，致畸因素似乎仅是一种IgG；在另一些血清中，致畸性不仅由一种IgG引起，还由其他致畸因素引起；在最后一组中，致畸因素似乎根本不是一种IgG。
