Department of Cardiology, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou 510515, China.
Cardiovasc Res. 2011 Jan 1;89(1):175-83. doi: 10.1093/cvr/cvq269. Epub 2010 Aug 23.
in this study, we attempted to detect a recent myocardial ischaemic event using ultrasound molecular imaging (UMI) with microbubbles (MB) targeted to intercellular adhesion molecule-1 (ICAM-1) in the late phase of reperfusion.
we created a myocardial ischaemia-reperfusion model in 60 C57/BL male mice to simulate an angina attack (ischaemia for 15 min, reperfusion for 1-24 h). The degree of myocardial inflammation and levels of ICAM-1 protein were determined by histological and immunohistochemical analyses. UMI with MB targeted to endothelial ICAM-1, as well as routine non-invasive methods including electrocardiography, echocardiography, and plasma troponin I levels, were utilized to evaluate ischaemia over the time course of reperfusion. Levels of ICAM-1 in the vascular endothelium were significantly increased over the time course of reperfusion (8-24 h) of the ischaemic myocardium. The video intensity of ICAM-1 molecular images of the ischaemic anterior wall was almost three times greater than that in the non-ischaemic posterior wall during the late phase (8-24 h) of reperfusion. In contrast, routine methods yielded only weak evidence of ischaemia.
UMI with MB targeted to endothelial ICAM-1 provides reliable evidence of a recent myocardial ischaemic event in the late phase of reperfusion.
在本研究中，我们试图通过靶向细胞间黏附分子-1（ICAM-1）的微泡（MB）超声分子成像（UMI）在再灌注晚期检测近期心肌缺血事件。
我们在 60 只 C57/BL 雄性小鼠中创建了心肌缺血再灌注模型，以模拟心绞痛发作（缺血 15 分钟，再灌注 1-24 小时）。通过组织学和免疫组织化学分析确定心肌炎症程度和 ICAM-1 蛋白水平。使用靶向内皮细胞 ICAM-1 的 MB 的 UMI 以及常规的非侵入性方法，包括心电图、超声心动图和血浆肌钙蛋白 I 水平，来评估再灌注过程中的缺血情况。缺血心肌再灌注 8-24 小时过程中，血管内皮细胞中 ICAM-1 的水平显著增加。在再灌注晚期（8-24 小时），缺血前壁的 ICAM-1 分子图像的视频强度几乎是非缺血后壁的三倍。相比之下，常规方法仅提供了缺血的微弱证据。
靶向内皮细胞 ICAM-1 的 MB 的 UMI 为再灌注晚期近期心肌缺血事件提供了可靠的证据。
