Kumar T R, Prakash V, Muralidhar K
Department of Zoology, University of Delhi, India.
J Protein Chem. 1990 Dec;9(6):727-33. doi: 10.1007/BF01024767.
Physicochemical and immunological properties of buffalo pituitary lutropin (buffalo LH) are reported here. The preparation was shown to be homogeneous by several physicochemical criteria. The molecular weight was found to be 30,000-40,000 by SDS-PAGE, GP-HPLC, and ultracentrifugation analyses. It showed certain interesting features, such as anomalous sedimentation behavior, microheterogeneity due to sugar-linked sulfate, and weak immunogenicity in rabbits. The subunit nature of the hormone has been confirmed. Sugar composition showed similarities as well as differences with LH of other species. Preliminary data on the homologous and heterologous RIAs, using iodinated sheep LH and buffalo LH and the respective antisera, have also been given.
本文报道了水牛垂体促黄体素（水牛促黄体激素）的物理化学和免疫学特性。通过多种物理化学标准表明该制剂是均一的。通过SDS-PAGE、凝胶渗透高效液相色谱（GP-HPLC）和超速离心分析，发现其分子量为30,000 - 40,000。它表现出某些有趣的特征，如异常沉降行为、糖基化硫酸化导致的微不均一性以及在兔体内的弱免疫原性。该激素的亚基性质已得到证实。糖组成与其他物种的促黄体激素既有相似之处也有差异。还给出了使用碘化绵羊促黄体激素和水牛促黄体激素以及各自抗血清进行同源和异源放射免疫分析（RIA）的初步数据。
