Weise H C, Graesslin D, Lichtenberg V, Rinne G
FEBS Lett. 1983 Aug 8;159(1-2):93-6. doi: 10.1016/0014-5793(83)80423-2.
The complete microheterogeneous system of human pituitary lutropin was demonstrated by gel isoelectric focusing. In addition 7 LH isohormones could be isolated by preparative column focusing and characterized with respect to physicochemical, biological and immunological properties. The in vivo biopotencies ranged from 4.50-11.50 IU/mg, the in vitro bioactivity was from 1.20-10.10 IU/mg, and the immunological activity was from 3.10-7.55 IU/mg. The sialic acid content was found to be 1.8-3.2%. Treatment with neuraminidase resulted in a shift of all bands to the alkaline region, however the 7 LH forms were still present.
通过凝胶等电聚焦证明了人垂体促黄体生成素的完整微不均一系统。此外，通过制备性柱聚焦可分离出7种促黄体生成素同工激素，并对其理化、生物学和免疫学特性进行了表征。其体内生物效价范围为4.50 - 11.50 IU/mg，体外生物活性为1.20 - 10.10 IU/mg，免疫活性为3.10 - 7.55 IU/mg。发现唾液酸含量为1.8 - 3.2%。用神经氨酸酶处理导致所有条带向碱性区域移动，但7种促黄体生成素形式仍然存在。
