将放射性35SO4(2-)掺入免疫反应性垂体促黄体激素中。

Incorporation of radioactive 35SO4(2-) into immunoreactive pituitary lutropin.

作者信息

Rajyalakshmi M, Arunasmithasri S, Srinivas V, Muralidhar K

出版信息

FEBS Lett. 1983 Apr 18;154(2):301-4. doi: 10.1016/0014-5793(83)80171-9.

Abstract

The terminal hexosamines of bovine pituitary lutropin are thought to contain a sulfate moiety. In order to test this, a biosynthetic approach was adopted. When rat and buffalo (bovine) pituitaries were incubated with radioactive 35SO4(2-) for 2 h in vitro it was observed that radioactivity test incorporated into trichloroacetic acid-precipitable proteins. When the radioactive proteins were treated with an anti-sheep lutropin serum, radioactivity was found in the immunoprecipitate. The incorporation into rat lutropin like material was very marginal while it was very significant in the case of buffalo lutropin.

摘要

牛垂体促黄体生成素的末端己糖胺被认为含有一个硫酸基团。为了验证这一点，采用了一种生物合成方法。当将大鼠和水牛（牛）垂体在体外与放射性35SO4(2-)一起孵育2小时时，观察到放射性被掺入到三氯乙酸可沉淀的蛋白质中。当用抗羊促黄体生成素血清处理放射性蛋白质时，在免疫沉淀物中发现了放射性。放射性掺入大鼠促黄体生成素样物质的量非常少，而在水牛促黄体生成素的情况下则非常显著。