[在血红蛋白多态性研究中使用反相高效液相色谱法分离非人血红蛋白]
[Separation using RP-HPLC of non-human globin in the study of hemoglobin polymorphism].
作者信息
Manca L, Cocco E, Naitana S, Masala B
机构信息
Istituto di Fisiologia Generale e Chimica Biologica, Università di Sassari.
出版信息
Boll Soc Ital Biol Sper. 1990 Sep;66(9):835-9.
PMID:2073384
Abstract
The successful extension of the reversed-phase HPLC methodology to the study of non-human globin chains is shown. A large-pore C4 column, and simple acetonitrile in aqueous TFA gradients, were used to completely separate and quantitate globin chains from caprines, swines, rodents, and birds. Results indicate a large hemoglobin polymorphism in some species.
摘要
结果表明，反相高效液相色谱法成功扩展到非人类珠蛋白链的研究。使用大孔C4柱和简单的乙腈在含三氟乙酸的水溶液中的梯度洗脱，可完全分离并定量测定来自山羊、猪、啮齿动物和鸟类的珠蛋白链。结果表明某些物种存在大量血红蛋白多态性。
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