用反相高效液相色谱法快速分离正常人及地中海贫血患者的人血红蛋白链。

Rapid separation of human globin chains in normal and thalassemia patients by RP-HPLC.

机构信息

Department of Biochemistry, Pharmacy School, Kermanshah University of Medical Sciences, Daneshgah Avenue, PO Box 67148-69914, Kermanshah, Iran.

出版信息

Mol Biol Rep. 2011 Jun;38(5):3213-8. doi: 10.1007/s11033-010-9994-4. Epub 2010 Mar 5.

DOI:10.1007/s11033-010-9994-4

PMID:20204523

Abstract

The present study describes a rapid and sensitive high-performance liquid chromatography (HPLC) method for the detection of human globin chains in blood. The method involves direct injection of globin chains which prepared by a standard method onto a micro bondapack C18 reversed-phase column (7.8 mm I.D.) with UV detection at 280 nm. The detection limit of hemoglobin (Hb) was 0.1 μg, which is equivalent to about 1 ml of fresh whole blood. We report here the rapid procedure for globin chain analysis. The present method will be useful for the determination of globin chain analysis in clinical laboratories, as well as in thalassemia and sickle cell disease patients.

摘要

本研究描述了一种快速灵敏的高效液相色谱（HPLC）法，用于检测血液中的人球蛋白链。该方法涉及通过标准方法直接将球蛋白链注入微键合 C18 反相柱（7.8mm ID）中，并用 280nm 的紫外检测。血红蛋白（Hb）的检测限为 0.1μg，相当于约 1ml 新鲜全血。我们在此报告球蛋白链分析的快速程序。本方法将有助于临床实验室以及地中海贫血和镰状细胞病患者的球蛋白链分析的测定。