The Key Laboratory of Molecular Animal Nutrition, College of Animal Science, Hua Jia Chi Campus, Zhejiang University, 164 Qiu Tao North Road, 310029 Hangzhou, China.
Biol Trace Elem Res. 2011 Sep;142(3):546-56. doi: 10.1007/s12011-010-8824-9. Epub 2010 Aug 24.
Three hundred and sixty healthy Ross × Ross 1-day-old broilers were used to study the effects of zinc glycine chelate (Zn-Gly) on oxidative stress, contents of trace elements, and intestinal morphology. All broilers were randomly assigned to six treatment groups, which replicates three times. Diets were as follows: (1) control (containing 29.3 mg zinc (Zn)/kg basic diet (0-21 days) and 27.8 mg Zn/kg (22-42 days)); (2) basic diet plus 30 mg Zn/kg from Zn-Gly; (3) basic diet plus 60 mg Zn/kg from Zn-Gly; (4) basic diet plus 90 mg Zn/kg from Zn-Gly; (5) basic diet plus 120 mg Zn/kg from Zn-Gly; and (6) positive control, basic diet plus 120 mg Zn/kg from zinc sulfate (ZnSO(4)). The results showed that the addition of 90 or 120 mg/kg Zn-Gly led to an improvement of activity of Cu/Zn superoxide dismutase and glutathione peroxidase and a reduction of malondialdehyde content in livers at 21 and 42 days. With 90 mg/kg Zn-Gly, the content of sera zinc increased by 17.55% (P < 0.05) in 21-day broilers and 10.77% (P > 0.05) in 42-day broilers compared with that of the control. Adding 120 mg/kg Zn-Gly or ZnSO(4) to broilers' diets greatly enhanced the content of zinc in feces at 21 days (P < 0.05) and at 42 days (P < 0.05). For 42-day chickens, increased villus height and decreased crypt depth of the jejunum could be observed in the second growth stage of broilers fed with 90 mg/kg Zn-Gly. Also, intestinal wall thickness decreased (P < 0.05). In addition, adding 90 mg/kg Zn-Gly to the diet markedly elevated villus length of duodenum and decreased crypt depth of ileum (P < 0.05) in 42-day broilers.
三百六十只健康的 Ross × Ross 1 日龄肉鸡用于研究甘氨酸锌螯合物 (Zn-Gly) 对氧化应激、微量元素含量和肠道形态的影响。所有肉鸡均随机分为六组处理，每组重复三次。日粮如下：(1) 对照组（0-21 天基础日粮含 29.3 mg 锌 (Zn)/kg，22-42 天含 27.8 mg Zn/kg）；(2) 基础日粮加 30 mg/kg Zn-Gly 中的 Zn；(3) 基础日粮加 60 mg/kg Zn-Gly 中的 Zn；(4) 基础日粮加 90 mg/kg Zn-Gly 中的 Zn；(5) 基础日粮加 120 mg/kg Zn-Gly 中的 Zn；(6) 阳性对照组，基础日粮加 120 mg/kg ZnSO(4) 中的 Zn。结果表明，添加 90 或 120 mg/kg Zn-Gly 可提高 21 和 42 日龄鸡肝脏中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，并降低丙二醛含量。添加 90 mg/kg Zn-Gly 可使 21 日龄鸡血清锌含量增加 17.55%（P<0.05），42 日龄鸡血清锌含量增加 10.77%（P>0.05）。在 21 日龄（P<0.05）和 42 日龄（P<0.05）时，添加 120 mg/kg Zn-Gly 或 ZnSO(4) 可显著提高粪便中锌的含量。对于 42 日龄的鸡，添加 90 mg/kg Zn-Gly 可使肉鸡第二生长阶段的空肠绒毛高度增加，隐窝深度降低（P<0.05），肠壁厚度降低（P<0.05）。此外，添加 90 mg/kg Zn-Gly 可显著提高 42 日龄肉鸡十二指肠绒毛长度，降低回肠隐窝深度（P<0.05）。
