Johnson Mark A, Kost Benedikt
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
Methods Mol Biol. 2010;655:155-76. doi: 10.1007/978-1-60761-765-5_11.
Pollen tubes grow rapidly in a strictly polarized manner as they transport male reproductive cells through female flower tissues to bring about fertilization. Vegetative pollen tube cells are an excellent model system to investigate processes underlying directional cell expansion. In this chapter, we describe materials and methods required for (1) the identification of novel factors essential for polarized cell growth through the isolation and analysis of Arabidopsis mutants with defects in pollen tube growth and (2) the detailed functional characterization of pollen tube proteins based on transient transformation and microscopic analysis of cultured tobacco pollen tubes.
花粉管以严格的极性方式快速生长，因为它们通过雌蕊组织运输雄配子以实现受精。营养花粉管细胞是研究定向细胞扩张过程的理想模型系统。在本章中，我们描述了以下所需的材料和方法：（1）通过分离和分析花粉管生长有缺陷的拟南芥突变体，鉴定极性细胞生长所必需的新因子；（2）基于瞬时转化和培养烟草花粉管的显微镜分析，对花粉管蛋白进行详细的功能表征。
