Isono Erika, Schwechheimer Claus
Department of Plant Systems Biology, Technical University Munich-Weihenstephan, Munich, Germany.
Methods Mol Biol. 2010;655:377-87. doi: 10.1007/978-1-60761-765-5_25.
Knowledge about the identity of the interacting partners is important for the understanding of the function and the cellular activity of a given protein. Here we describe co-immunoprecipitation and pull-down as methods that are widely used for the identification and characterization of protein-protein interactions. These methods are well suited to find or confirm the interaction among multiple proteins, given the availability of a specific antibody for or a tagged version of the protein of interest.
了解相互作用伙伴的身份对于理解特定蛋白质的功能和细胞活性很重要。在这里，我们将免疫共沉淀和下拉法描述为广泛用于鉴定和表征蛋白质-蛋白质相互作用的方法。鉴于有针对感兴趣蛋白质的特异性抗体或其标记版本，这些方法非常适合于发现或确认多种蛋白质之间的相互作用。
