Dubois Charlotte, Prevarskaya Natalia
INSERM U1003, Laboratoire d'Excellence, Canaux Ioniques d'Intérêt Thérapeutique, Équipe Labellisée Par la Ligue Nationale Contre le Cancer, SIRIC ONCOLille, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Methods Mol Biol. 2018;1843:107-113. doi: 10.1007/978-1-4939-8704-7_9.
The characterization of protein-protein interactions through methods such as co-immunoprecipitation, followed by Western blot analysis, is a crucial step in the understanding of protein functions and the biology of the cell. Since the discovery of ORAI and STIM proteins as component of store-operated channel (SOC), overexpressing systems have been used to demonstrate how ORAI and STIM can associate with physiological and pathological conditions. Here we describe a protocol allowing endogenous studies.
通过免疫共沉淀等方法，随后进行蛋白质印迹分析来表征蛋白质-蛋白质相互作用，是理解蛋白质功能和细胞生物学的关键步骤。自从发现ORAI和STIM蛋白作为储存式钙通道（SOC）的组成成分以来，过表达系统已被用于证明ORAI和STIM如何与生理和病理状况相关联。在此，我们描述一种允许进行内源性研究的方案。
