State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, People's Republic of China.
Arch Insect Biochem Physiol. 2010 Oct;75(2):121-38. doi: 10.1002/arch.20386.
Ribosomal protein L17 (RPL17) is a core protein of the large (60S) ribosomal subunit and plays an important role in protein synthesis. In this report, a RPL17 gene was isolated from Apis cerana cerana, designated as AccRPL17. Alignment analysis showed that AccRPL17 exhibits high homology to other known RPL17s. Moreover, genomic sequence analysis revealed that five exons are splitted by four introns, and the position of the first intron is comparatively conservative, being localized in the 5' untranslated region. Partial putative cis-acting elements related to development were also examined. Quantitative real-time PCR showed that the highest mRNA level was detected in larvae on the fifth day. Simultaneously, immunohistochemical localization showed that AccRPL17 is primarily concentrated in muscular tissues, stigma, body wall, and the surrounding of the eye in the fifth-instar larvae. Further studies suggested that AccRPL17 might be involved in responses to abiotic stresses. This is a report attempting to analyze the expression and distribution of RPL17 in A. cerana cerana. These results indicated that AccRPL17 might play an important role in insect development, and the importance of AccRPL17 in participating in abiotic stresses is discussed.
核糖体蛋白 L17（RPL17）是大亚基（60S）核糖体的核心蛋白，在蛋白质合成中发挥重要作用。本研究从中华蜜蜂（Apis cerana cerana）中分离得到一个 RPL17 基因，命名为 AccRPL17。序列比对分析表明，AccRPL17 与其他已知的 RPL17 具有高度同源性。此外，基因组序列分析显示该基因含有 5 个外显子和 4 个内含子，第一个内含子的位置比较保守，位于 5'非翻译区。还对部分与发育相关的顺式作用元件进行了预测。实时定量 PCR 结果显示，AccRPL17 在 5 日龄幼虫中的表达量最高。免疫组化定位显示，AccRPL17 主要集中在 5 日龄幼虫的肌肉组织、触角、体壁和眼周。进一步的研究表明，AccRPL17 可能参与了非生物胁迫的响应。本研究首次对 RPL17 在中华蜜蜂中的表达和分布进行了分析，结果表明 AccRPL17 可能在昆虫发育过程中发挥重要作用，并探讨了 AccRPL17 参与非生物胁迫响应的重要性。
