Danchenko E O, Chirkin A A
Sud Med Ekspert. 2010 May-Jun;53(3):25-8.
A simple specific method for the detection of glycogen concentration in various tissues without disintegration of its structure is described. The method includes staining of glycogen molecules with iodine. The results of determination do not depend on the presence of polysaccharides because they are not stained with iodine but release reducing sugars during hydrolysis. Glycogen content is calculated from the calibration curve.
描述了一种简单的特定方法，用于在不破坏其结构的情况下检测各种组织中的糖原浓度。该方法包括用碘对糖原分子进行染色。测定结果不依赖于多糖的存在，因为多糖不会被碘染色，但在水解过程中会释放还原糖。糖原含量根据校准曲线计算得出。
