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Genotyping of Aeromonas hydrophila by box elements.

作者信息

Singh Vijai, Chaudhary Dharmendra Kumar, Mani Indra, Somvanshi Pallavi, Rathore Gaurav, Sood Neeraj

机构信息

National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226002, India.

出版信息

Mikrobiologiia. 2010 May-Jun;79(3):390-3.

PMID:20734813
Abstract

PCR-based DNA fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates of Aeromonas hydrophila. PCR-based fingerprinting method has an advantage of having repetitive sequence also called Box elements that are interspersed throughout the genome in diverse bacterial species. The BOX-PCR fingerprinting technique was evaluated for the discrimination of different isolates of A. hydrophila. All the studied isolates have shown major banding patterns ranged from 500-3000 bp. These finding could be advantageous to investigate the strain level specific fingerprints of A. hydrophila as potential genotypic markers.

摘要

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