Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Langmuir. 2010 Sep 21;26(18):14409-13. doi: 10.1021/la1027674.
We report integrated video and total internal reflection microscopy measurements of protein stabilized 110 nm Au nanoparticles confined in 280 nm gaps in physiological media. Measured potential energy profiles display quantitative agreement with Brownian dynamic simulations that include hydrodynamic interactions and camera exposure time and noise effects. Our results demonstrate agreement between measured nonspecific van der Waals and adsorbed protein interactions with theoretical potentials. Confined, lateral nanoparticle diffusivity measurements also display excellent agreement with predictions. These findings provide a basis to interrogate specific biomacromolecular interactions in similar experimental configurations and to design future improved measurement methods.
我们报告了在生理介质中 280nm 间隙内限制的蛋白质稳定的 110nmAu 纳米粒子的集成视频和全内反射显微镜测量。测量的势能曲线与包括流体动力学相互作用以及相机曝光时间和噪声影响的布朗动力学模拟定量吻合。我们的结果表明，测量的非特异性范德华力和吸附蛋白质相互作用与理论势能之间存在一致性。受限的侧向纳米粒子扩散率测量也与预测结果非常吻合。这些发现为在类似实验配置中探究特定生物大分子相互作用以及设计未来改进的测量方法提供了基础。
