The Swire Institute of Marine Science, School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, PR China.
Nanomedicine (Lond). 2010 Aug;5(6):951-61. doi: 10.2217/nnm.10.59.
Double-walled carbon nanotubes (DWNTs) are found in a variety of consumer products, but there are no ecotoxicity data of DWNTs into marine organisms.
MATERIALS & METHODS: Chronic toxicity of DWNTs was investigated with the diatom Thalassiosira pseudonana, copepod Tigriopus japonicus and medaka Oryzias melastigma. DWNTs were dispersed using sonication (so-DWNTs) and stirring (st-DWNTs) for comparison.
The median aggregation size (0.89 microm(2)) of so-DWNTs was smaller than that of st-DWNTs (21.8 microm(2)). Exposure to DWNTs led to growth inhibition of T. pseudonana with EC(50)s of 1.86 and 22.7 mg/l for so- and st-DWNTs, respectively. Population growth of T. japonicus was reduced to 0.1 mg/l for so-DWNTs and 10 mg/l for st-DWNTs. Growth inhibition in O. melastigma was observed at 10 mg/l for so-DWNTs but not for st-DWNTs.
Given that so-DWNTs are consistently significantly more toxic than st-DWNTs, dispersion method and size of aggregations should be considered in DWNT toxicity testing.
双壁碳纳米管（DWNTs）存在于各种消费产品中，但目前尚无 DWNTs 对海洋生物的生态毒性数据。
采用海洋硅藻新月菱形藻、桡足类动物 T. japonicus 和斑马鱼 O. melastigma 研究了 DWNTs 的慢性毒性。通过超声（so-DWNTs）和搅拌（st-DWNTs）分散 DWNTs 进行比较。
so-DWNTs 的中位聚集粒径（0.89 µm²）小于 st-DWNTs（21.8 µm²）。DWNTs 的暴露导致新月菱形藻的生长抑制，so-DWNTs 和 st-DWNTs 的 EC50 分别为 1.86 和 22.7 mg/L。so-DWNTs 使 T. japonicus 的种群增长率降低至 0.1 mg/L，st-DWNTs 则降低至 10 mg/L。so-DWNTs 的浓度为 10 mg/L 时观察到斑马鱼的生长抑制，但 st-DWNTs 则没有。
鉴于 so-DWNTs 的毒性始终明显大于 st-DWNTs，在 DWNT 毒性测试中应考虑分散方法和聚集粒径。
