双壁碳纳米管对三种海洋生物的慢性毒性：不同分散方法的影响。

Chronic toxicity of double-walled carbon nanotubes to three marine organisms: influence of different dispersion methods.

机构信息

The Swire Institute of Marine Science, School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, PR China.

出版信息

Nanomedicine (Lond). 2010 Aug;5(6):951-61. doi: 10.2217/nnm.10.59.

DOI:10.2217/nnm.10.59

PMID:20735229

Abstract

AIM

Double-walled carbon nanotubes (DWNTs) are found in a variety of consumer products, but there are no ecotoxicity data of DWNTs into marine organisms.

MATERIALS & METHODS: Chronic toxicity of DWNTs was investigated with the diatom Thalassiosira pseudonana, copepod Tigriopus japonicus and medaka Oryzias melastigma. DWNTs were dispersed using sonication (so-DWNTs) and stirring (st-DWNTs) for comparison.

RESULTS

The median aggregation size (0.89 microm(2)) of so-DWNTs was smaller than that of st-DWNTs (21.8 microm(2)). Exposure to DWNTs led to growth inhibition of T. pseudonana with EC(50)s of 1.86 and 22.7 mg/l for so- and st-DWNTs, respectively. Population growth of T. japonicus was reduced to 0.1 mg/l for so-DWNTs and 10 mg/l for st-DWNTs. Growth inhibition in O. melastigma was observed at 10 mg/l for so-DWNTs but not for st-DWNTs.

CONCLUSION

Given that so-DWNTs are consistently significantly more toxic than st-DWNTs, dispersion method and size of aggregations should be considered in DWNT toxicity testing.

摘要

目的

双壁碳纳米管（DWNTs）存在于各种消费产品中，但目前尚无 DWNTs 对海洋生物的生态毒性数据。

材料与方法

采用海洋硅藻新月菱形藻、桡足类动物 T. japonicus 和斑马鱼 O. melastigma 研究了 DWNTs 的慢性毒性。通过超声（so-DWNTs）和搅拌（st-DWNTs）分散 DWNTs 进行比较。

结果

so-DWNTs 的中位聚集粒径（0.89 µm²）小于 st-DWNTs（21.8 µm²）。DWNTs 的暴露导致新月菱形藻的生长抑制，so-DWNTs 和 st-DWNTs 的 EC50 分别为 1.86 和 22.7 mg/L。so-DWNTs 使 T. japonicus 的种群增长率降低至 0.1 mg/L，st-DWNTs 则降低至 10 mg/L。so-DWNTs 的浓度为 10 mg/L 时观察到斑马鱼的生长抑制，但 st-DWNTs 则没有。