Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Asansi, Chungnam, Korea.
FEMS Microbiol Lett. 2010 Oct;311(2):119-25. doi: 10.1111/j.1574-6968.2010.02075.x. Epub 2010 Aug 23.
Neocarzinostatin (NCS) is an enediyne antibiotic produced by Streptomyces carzinostaticus. The NCS chromophore consists of an enediyne core, a sugar moiety, and a naphthoic acid (NA) moiety. The latter plays a key role in binding the NCS chromophore to its apoprotein to protect and stabilize the bioactive NCS chromophore. In this study, we expressed three genes: ncsB (naphthoic acid synthase), ncsB3 (P450 hydroxylase), and ncsB1 (O-methyltransferase), in Streptomyces lividans TK24. The three genes were sufficient to produce 2-hydroxy-7-methoxy-5-methyl-1-naphthoic acid. Production was analyzed and confirmed by LC-MS and nuclear magnetic resonance. Here, we report the functional characterization of ncsB3 and thereby elucidate the complete biosynthetic pathway of NA moiety of the NCS chromophore.
新制癌菌素（NCS）是一种由链霉菌产生的烯二炔抗生素。NCS 生色团由一个烯二炔核心、一个糖部分和一个萘甲酸（NA）部分组成。后者在将 NCS 生色团与其脱辅基蛋白结合以保护和稳定生物活性 NCS 生色团方面起着关键作用。在这项研究中，我们在链霉菌 lividans TK24 中表达了三个基因：ncsB（萘甲酸合酶）、ncsB3（P450 羟化酶）和 ncsB1（O-甲基转移酶）。这三个基因足以产生 2-羟基-7-甲氧基-5-甲基-1-萘甲酸。通过 LC-MS 和核磁共振分析和证实了生产。在这里，我们报告了 ncsB3 的功能特征，从而阐明了 NCS 生色团的 NA 部分的完整生物合成途径。
