Institut National de la Santwe et de la Recherche Medicale, Paris, France.
Emerg Infect Dis. 2010 Sep;16(9):1349-56. doi: 10.3201/eid1609.091389.
Klebsiella pneumoniaeisolates that produce carbapenemases (KPCs) are rapidly disseminating worldwide. To determine their genetic background, we investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The isolates were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional Beta-lactamase content. They harbored a naturally chromosome-encoded bla gene (blaSHV-1 [12.5%], blaSHV-11 [68.7%], or blaOKP-AVB [18.8%]) and several acquired and plasmid-encoded genes (blaTEM-1 [81.3%], blaCTX-M-2 [31.3%], blaCTX-M-12 [12.5%], blaCTX-M-15 [18.7%], and blaOXA-9 [37.5%]). The blaKPC-2 gene was always associated with 1 of the Tn4401 isoforms (a, b, or c). Tn4401 was inserted on different-sized plasmids that belonged to different incompatibility groups. Several blaKPC-containing K. pneumoniae clones were found: 9 different pulsotypes with 1 major (sequence type 258) and 7 minor distinct allelic profiles. Different clones harboring different plasmids but having identical genetic structure, Tn4401, could be at the origin of the worldwide spread of this emerging resistance gene.
产碳青霉烯酶（KPCs）的肺炎克雷伯菌分离株正在全球范围内迅速传播。为了确定其遗传背景，我们研究了来自 5 个国家的 16 株携带 blaKPC-2 的肺炎克雷伯菌分离株。这些分离株具有多药耐药性，携带 blaKPC-2 基因，并且具有不同的β-内酰胺酶含量。它们携带一个天然染色体编码的 bla 基因（blaSHV-1[12.5%]、blaSHV-11[68.7%]或 blaOKP-AVB[18.8%]）和几个获得的和质粒编码的基因（blaTEM-1[81.3%]、blaCTX-M-2[31.3%]、blaCTX-M-12[12.5%]、blaCTX-M-15[18.7%]和 blaOXA-9[37.5%]）。blaKPC-2 基因总是与 Tn4401 的 1 个亚型（a、b 或 c）相关联。Tn4401 插入到属于不同不相容群的不同大小的质粒上。发现了几种携带 blaKPC 的肺炎克雷伯菌克隆：9 个不同的脉冲型，1 个主要（序列类型 258）和 7 个次要的不同等位基因谱。携带不同质粒但具有相同遗传结构的不同克隆，Tn4401，可能是这种新兴耐药基因在全球传播的起源。
