竹笋中酚酸与细胞壁多糖的连接

Linkage of phenolic acids to cell-wall polysaccharides of bamboo shoot.

作者信息

Ishii T, Hiroi T

机构信息

Forestry and Forest Products Research Institute, Ibaraki, Japan.

出版信息

Carbohydr Res. 1990 Oct 10;206(2):297-310. doi: 10.1016/0008-6215(90)80069-f.

DOI:10.1016/0008-6215(90)80069-f

PMID:2073638

Abstract

Hydrolysis of bamboo shoot cell walls with Driselase (a fungal enzyme preparation) gave xyloglucan and arabinoxylan oligosaccharides containing ferulic and p-coumaric acids, respectively. The structures of two oligosaccharides containing phenolic acids are here determined to be O-(4-O-trans-feruloyl-alpha-D-xylopyranosyl)-(1----6)-D-glucopy rano se and O-[5-O-(trans-p-coumaroyl(-alpha-L-arabinofuranosyl]-(1----3)-O-be ta-D-xylopyranosyl-(1----4)-D-xylopyranose, on the basis of n.m.r. spectroscopy, methylation analysis, and f.a.b.-m.s. The possible role of phenolic acid substituents in cell-wall architecture is discussed.

摘要

用Driselase（一种真菌酶制剂）水解竹笋细胞壁，分别得到了含有阿魏酸和对香豆酸的木葡聚糖和阿拉伯木聚糖低聚糖。基于核磁共振光谱、甲基化分析和快原子轰击质谱，确定了两种含酚酸的低聚糖结构分别为O-(4-O-反式-阿魏酰基-α-D-吡喃木糖基)-(1→6)-D-吡喃葡萄糖和O-[5-O-(反式-对香豆酰基-α-L-阿拉伯呋喃糖基]-(1→3)-O-β-D-吡喃木糖基-(1→4)-D-吡喃木糖。文中讨论了酚酸取代基在细胞壁结构中的可能作用。