Smith B G, Harris P J
School of Biological Sciences, The University of Auckland, New Zealand.
Phytochemistry. 2001 Mar;56(5):513-9. doi: 10.1016/s0031-9422(00)00401-5.
The ester-linkage of ferulic acid (mainly E) to polysaccharides in primary cell walls of pineapple fruit (Ananas comosus) (Bromeliaceae) was investigated by treating a cell-wall preparation with 'Driselase' which contains a mixture of endo- and exo-glycanases, but no hydroxycinnamoyl esterase activity. The most abundant feruloyl oligosaccharide released was O-5-O-(E-feruloyl)-alpha-L-arabinofuranosyl-O-beta-D-xylopyranosyl-(1-->4)-D-xylopyranose (FAXX). This indicated that the ferulic acid is ester-linked to glucuronoarabinoxylans in the same way as in the primary walls of grasses and cereals (Poaceae). Glucuronoarabinoxylans are the major non-cellulosic polysaccharides in the pineapple cell walls.
通过用含有内切和外切聚糖酶混合物但无羟基肉桂酸酯酶活性的“溶菌酶”处理细胞壁制剂，研究了阿魏酸（主要是E型）与菠萝果实（凤梨科凤梨属）初生细胞壁中多糖的酯键连接。释放出的最丰富的阿魏酰寡糖是O-5-O-(E-阿魏酰基)-α-L-阿拉伯呋喃糖基-O-β-D-木吡喃糖基-(1→4)-D-木吡喃糖（FAXX）。这表明阿魏酸与葡糖醛酸阿拉伯木聚糖的酯键连接方式与禾本科和谷类作物（禾本科）的初生细胞壁相同。葡糖醛酸阿拉伯木聚糖是菠萝细胞壁中主要的非纤维素多糖。
