Department of Anesthesiology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Anesth Analg. 2010 Nov;111(5):1117-21. doi: 10.1213/ANE.0b013e3181f3351a. Epub 2010 Aug 24.
We tested the hypothesis that caveolin-3 (Cav-3) is essential for opioid-induced preconditioning in vivo. Cav-3 overexpressing mice, Cav-3 knockout mice, and controls were exposed to myocardial ischemia/reperfusion (I/R) in the presence of SNC-121 (SNC), a δ-selective opioid agonist, or naloxone, a nonselective opioid antagonist. Controls were protected from I/R injury by SNC. No protection was produced by SNC in Cav-3 knockout mice. Cav-3 overexpressing mice showed innate protection from I/R compared with controls that was abolished by naloxone. Our results show that opioid-induced preconditioning is dependent on Cav-3 expression and that endogenous protection in Cav-3 overexpressing mice is opioid dependent.
我们检验了这样一个假设,即陷窝蛋白-3(Cav-3)对于体内阿片类药物预处理是必需的。在存在 SNC-121(一种 δ 选择性阿片类激动剂)或纳洛酮(一种非选择性阿片类拮抗剂)的情况下,过表达 Cav-3 的小鼠、Cav-3 敲除小鼠和对照小鼠暴露于心肌缺血/再灌注(I/R)中。对照小鼠通过 SNC 受到 I/R 损伤的保护。SNC 对 Cav-3 敲除小鼠没有产生保护作用。与对照小鼠相比,过表达 Cav-3 的小鼠表现出对 I/R 的先天保护作用,而纳洛酮则消除了这种保护作用。我们的结果表明,阿片类药物预处理依赖于 Cav-3 的表达,并且过表达 Cav-3 的小鼠中的内源性保护作用依赖于阿片类药物。