Akhmatova N K, Chertov I V, Akhmatov E A, Kurbatova E A, Egorova N B, Semenova I B
Zh Mikrobiol Epidemiol Immunobiol. 2010 May-Jun(3):56-61.
Evaluation of differentiation dynamics and localization of various populations of lymphocytes as well as their expression of TLRs during different methods of administration (intranasal, oral, and subcutaneous) of bacterial ligands of opportunistic microorganisms (Immunovac-VP-4 vaccine) in experiment on mice.
Polycomponent vaccine Immunovac-VP-4 consisting of ligands of 4 opportunistic bacteria was administered to CBA line mice. Groups of mice were immunized orally, subcutaneously, or intranasally. Number of mononuclear leukocytes, as well as levels of cytokines, lymphocytic antigens, and cytotoxic activity of cells were measured.
It was demonstrated that modification of immunophenotype of lymphocytes and cytotoxic activity of NK cells depends from route of administration of Immunovac-VP-4 because the most intensive activation of cells occurred in organs proximal to place of vaccine application. However, already 1 day after administration of vaccine there was intensive exchange between lymphoid cells not only in nasal associated lymphoid tissue, bronchial associated lymphoid tissue, and gut associated lymphoid tissue but also in spleen that points to integration of fine mechanisms of mucosal and systemic immune response regulation.
Development of noninvasive methods of vaccination is an optimal way of safe and mass prevention of infectious diseases.
在小鼠实验中，评估机会性微生物（免疫疫苗-VP-4疫苗）的细菌配体通过不同给药方式（鼻内、口服和皮下）给药时，各种淋巴细胞群体的分化动力学、定位及其Toll样受体（TLR）的表达。
将由4种机会性细菌的配体组成的多组分疫苗免疫疫苗-VP-4给予CBA品系小鼠。将小鼠分组进行口服、皮下或鼻内免疫。检测单核白细胞数量以及细胞因子水平、淋巴细胞抗原和细胞的细胞毒性活性。
结果表明，淋巴细胞免疫表型和自然杀伤（NK）细胞的细胞毒性活性的改变取决于免疫疫苗-VP-4的给药途径，因为细胞的最强烈激活发生在疫苗接种部位附近的器官中。然而，在疫苗接种后1天，不仅鼻相关淋巴组织、支气管相关淋巴组织和肠道相关淋巴组织中的淋巴细胞之间，而且脾脏中的淋巴细胞之间就已经发生了强烈的交换，这表明黏膜和全身免疫反应调节的精细机制相互整合。
开发非侵入性疫苗接种方法是安全、大规模预防传染病的最佳途径。
