Akhmatova N K, Egorova N B, Akhmatov E A, Kurbatova E A, Semenova I B, Chertov I V, Semenov B F, Zverev V V
Zh Mikrobiol Epidemiol Immunobiol. 2010 Jan-Feb(1):50-4.
To determine level of Toll-like receptors (TLRs) expression in spleen and lymphatic nodes of mice after immunization by mucosal routes.
Mice were immunized with polycomponent vaccine Immunovac either by mucosal or subcutaneous route. Expression of TLRs in spleen, respiratory tract-associated lymphatic nodes as well as in small intestine was measured in immunized mice by flow cytomentry method.
After immunization of mice by subcutaneous, intranasal and oral routes level of TLRs expression was different. Significant expression of TLR9 and absence of TLR2 expression was noted after non-parenteral methods of immunization. After oral immunization expression of TLRs was identified in gut-and respiratory tract-associated lymphoid tissue as well as in spleen; after intranasal immunization--in respiratory tract-associated lymphoid tissue, and after subcutaneous immunization--in spleen and respiratory tract-associated lymphoid tissue.
After oral immunization expression of TLRs was identified in all studied organs, including spleen. Involvement of spleen to this process allows to assume establishment of not only local but also systemic immunity.
确定经黏膜途径免疫后小鼠脾脏和淋巴结中Toll样受体（TLRs）的表达水平。
用多组分疫苗免疫宝（Immunovac）通过黏膜或皮下途径对小鼠进行免疫。采用流式细胞术检测免疫小鼠脾脏、呼吸道相关淋巴结以及小肠中TLRs的表达。
经皮下、鼻内和口服途径免疫小鼠后，TLRs的表达水平有所不同。非胃肠外免疫方法后，TLR9有显著表达，而TLR2无表达。口服免疫后，在肠道和呼吸道相关淋巴组织以及脾脏中均检测到TLRs的表达；鼻内免疫后，在呼吸道相关淋巴组织中检测到表达；皮下免疫后，在脾脏和呼吸道相关淋巴组织中检测到表达。
口服免疫后，在包括脾脏在内的所有研究器官中均检测到TLRs的表达。脾脏参与这一过程表明不仅建立了局部免疫，还建立了全身免疫。
