Diehl V, Schaadt M, Kirchner H, Hellriegel K P, Gudat F, Fonatsch C, Laskewitz E, Guggenheim R
Blut. 1978 Jun 20;36(6):331-8. doi: 10.1007/BF01000590.
Two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (LCL). The 9-month-old plasma cell line showed the typical morphology of plasmoblasts. The cells neither had B- nor T-lymphocyte characterisitics, were EBV negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. The cytogenetic findings indicate a monoclonal proliferation of the plasmacells. No tumor growth in thymusless nude mice could be induced upon intracranial inoculation with these cells. In contrast, the autologous LCL, cultured after addition of exogenous EBV, showed the characteristic markers of lymphoblastoid cells, with the typical morphology of pear- and handmirror-shaped lymphoblasts, growing in clumps. They had C3- and Fc-receptors, surface-Ig, E-rosette-negativity, a diploid karyotype, and EBV dependent macromolecule synthesis. They lymphoblastoid cells produced intracranial tumors in nude mice in 8 out of 8 attempts.
一种是浆细胞增殖的细胞系，另一种是淋巴母细胞样细胞增殖（LCL）的细胞系。9个月大的浆细胞系表现出成浆细胞的典型形态。这些细胞既没有B淋巴细胞也没有T淋巴细胞特征，EBV阴性，并显示出包括14q +标记在内的各种标记染色体的非整倍体。细胞遗传学结果表明浆细胞的单克隆增殖。用这些细胞进行颅内接种后，在无胸腺裸鼠中未诱导出肿瘤生长。相反，添加外源性EBV后培养的自体LCL显示出淋巴母细胞样细胞的特征性标记，具有典型的梨形和手镜形淋巴母细胞形态，成簇生长。它们具有C3和Fc受体、表面免疫球蛋白、E玫瑰花结阴性、二倍体核型以及EBV依赖性大分子合成。在8次尝试中，淋巴母细胞样细胞在8只裸鼠中产生了颅内肿瘤。
