Fukukawa T, Hirano T, Sakaguchi N, Teranishi T, Tsuyuguchi I, Nagao N, Naito N, Yoshimura K, Okubo Y, Tohda H, Oikawa A
J Immunol. 1981 May;126(5):1697-701.
Cytotoxic T lymphocytes (CTL) against autologous EBV-transformed B lymphoblastoid cell line (LCL) were induced in vitro by culturing peripheral blood lymphocytes (PBL) of healthy donors together with mitomycin C-treated autologous LCL for 6 days. The cytotoxic cells developed only from the E-rosette-positive fraction but not from the negative fraction of PBL. These CTL killed autologous LCL but not PWM-stimulated autologous PBL. In addition, the CTL killed allogeneic LCL when at least 1 of the HLA-A antigens was identical with that of the LCL of CTL donor. However, identity of HLA-B and HLA-C antigens was not enough for a significant killing of allogeneic LCL. The specificity of the CTL was also confirmed by a cold target inhibition test. These results indicated that the CTL induced specifically recognized EBV-transformed cells with HLA restriction.
通过将健康供体的外周血淋巴细胞（PBL）与丝裂霉素C处理的自体B淋巴母细胞样细胞系（LCL）共同培养6天，在体外诱导出针对自体EBV转化的B淋巴母细胞样细胞系（LCL）的细胞毒性T淋巴细胞（CTL）。细胞毒性细胞仅从PBL的E花环阳性部分产生，而不是从阴性部分产生。这些CTL杀死自体LCL，但不杀死PWM刺激的自体PBL。此外，当至少一种HLA - A抗原与CTL供体的LCL的HLA - A抗原相同时，CTL杀死同种异体LCL。然而，HLA - B和HLA - C抗原的相同性不足以显著杀死同种异体LCL。CTL的特异性也通过冷靶抑制试验得到证实。这些结果表明，诱导的CTL特异性识别具有HLA限制性的EBV转化细胞。
