Henle G, Henle W
J Bacteriol. 1966 Mar;91(3):1248-56. doi: 10.1128/jb.91.3.1248-1256.1966.
Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91:1248-1256. 1966.-Indirect immunofluorescence tests led to the brilliant staining of a small proportion of the cells in five different cultures derived from Burkitt's (African) lymphomas. The reaction was not restricted to the 17 sera from cases of this disease but extended to many sera from American individuals, whether healthy donors or patients suffering from a variety of illnesses. The incidence of positive sera increased with age from about 30% in childhood to > 90% in adults. Fluorescein-isothiocyanate-conjugated human gamma-globulins were suitable for direct staining of the same proportion of cells. The stained cells appeared to be in varying stages of degeneration, but cultural conditions leading to an increase in the cellular death rates failed to result in a rise in fluorescent cells. Several observations suggest that the stainable cells might be those which are seen to harbor virus particles under the electron microscope. Two cell lines derived from leukemic patients in this country also contained a small fraction of stainable cells but two others, and numerous primary human leukocyte cultures, gave consistently negative results. Attempts to relate the staining to known viral antigens have failed to implicate herpes simplex, varicella, cytomegalo, and reo viruses types 1, 2, and 3. The nature of the virus carried by the lymphoma cells as well as of the staining reactions remains to be determined.
亨勒，格特鲁德（费城儿童医院，宾夕法尼亚州费城），以及维尔纳·亨勒。伯基特淋巴瘤来源细胞中的免疫荧光。《细菌学杂志》91:1248 - 1256。1966年。——间接免疫荧光试验使来自5种不同的伯基特（非洲）淋巴瘤培养物中的一小部分细胞呈现明亮染色。该反应不仅限于来自这种疾病患者的17份血清，还扩展到许多来自美国个体的血清，无论他们是健康献血者还是患有各种疾病的患者。阳性血清的发生率随年龄增长而增加，从儿童期的约30%增至成年人的>90%。异硫氰酸荧光素偶联的人γ球蛋白适用于对相同比例的细胞进行直接染色。染色的细胞似乎处于不同的退化阶段，但导致细胞死亡率增加的培养条件并未使荧光细胞数量增加。多项观察表明，可染色的细胞可能是在电子显微镜下可见含有病毒颗粒的细胞。在该国，源自白血病患者的2株细胞系也含有一小部分可染色细胞，但另外2株细胞系以及众多原代人白细胞培养物始终呈阴性结果。试图将这种染色与已知病毒抗原联系起来的尝试未能表明单纯疱疹病毒、水痘病毒、巨细胞病毒以及1型、2型和3型呼肠孤病毒与之有关。淋巴瘤细胞携带的病毒的性质以及染色反应的性质仍有待确定。
