Division of Natural Sciences, Thimann Laboratories, University of California, Santa Cruz, Santa Cruz, California 95064, USA.
Dev Biol. 1981 Jun;84(2):277-85. doi: 10.1016/0012-1606(81)90395-x.
The pattern of cuticle protein synthesis during development of the nematode Caenorhabditis elegans has been studied using NaH14CO3. Both pulse-labeling and pulse-chase-labeling experiments indicate that synthesis of cuticle components occurs at high levels during the molting periods and at much reduced rates during the intermolt periods. No such discontinuous pattern is observed for the synthesis of total noncuticle macromolecules during development. The soluble and insoluble proteins of the cuticle, which comprise the inner and outer cuticle layers, respectively, follow similar patterns of synthesis during the two molts examined. At each molt the structural components of the cuticle account for approximately 10% of the total macromolecules labeled by NaH14CO3. No evidence is found for reuse of cuticle material between successive developmental stages of C. elegans.
已经使用 NaH14CO3 研究了线虫秀丽隐杆线虫发育过程中表皮蛋白合成的模式。脉冲标记和脉冲追踪标记实验均表明，蜕皮期间表皮成分的合成水平很高，而在间期间则大大降低。在发育过程中，总非表皮大分子的合成没有这种不连续的模式。分别构成内表皮层和外表皮层的表皮的可溶性和不溶性蛋白质在研究的两个蜕皮期间的合成模式相似。在每次蜕皮中，表皮的结构成分约占经 NaH14CO3 标记的总大分子的 10%。在秀丽隐杆线虫的连续发育阶段之间没有发现表皮材料再利用的证据。
