Sydney Centre for Developmental and Regenerative Medicine, University of Sydney, St. Leonards, New South Wales, Australia.
Biol Reprod. 2010 Dec;83(6):958-64. doi: 10.1095/biolreprod.109.083162. Epub 2010 Aug 25.
The developmental viability of the preimplantation embryo requires the successful formation of a cluster of pluripotent stem cells called the inner cell mass. Development is variably compromised by a range of exogenous stressors (including their production by assisted reproductive technologies). Inbred C57BL/6 strain embryos are particularly susceptible to the stresses associated with embryo culture, whereas hybrid embryos are more resistant, and this is accounted for in part by the overexpression of transformation-related protein 53 in cultured inbred embryos compared with similarly treated hybrid embryos or embryos not subjected to culture. We show here that this loss of viability is a consequence of the Trp53-dependent reduction in the capacity of blastocysts to form a proliferating inner cell mass. Formation of the trophectodermal line was not adversely affected by these stresses.
胚胎植入前的发育活力需要成功形成称为内细胞团的多能干细胞簇。一系列外源应激源（包括辅助生殖技术产生的应激源）会不同程度地损害发育。近交 C57BL/6 品系胚胎特别容易受到与胚胎培养相关的应激的影响，而杂交胚胎则更具抗性，这部分归因于与经过类似处理的杂交胚胎或未进行培养的胚胎相比，培养的近交胚胎中转化相关蛋白 53 的过度表达。我们在这里表明，这种活力的丧失是 Trp53 依赖性的，导致囊胚形成增殖性内细胞团的能力降低。滋养外胚层的形成不受这些应激的不利影响。
