Bazzaz J T, Nazari M, Nazem H, Amiri P, Fakhrzadeh H, Heshmat R, Abbaszadeh S, Amoli M M
Endocrinology and Metabolism Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
J Postgrad Med. 2010 Jul-Sep;56(3):173-5. doi: 10.4103/0022-3859.68629.
Apolipoprotein E (APOE) is known as a major regulator of blood lipid levels in humans. A number of APOE gene allelic variants have been reported including E2, E3 and E4. Recent studies suggested a role for APOE in obesity and increased Body Mass Index (BMI) and plasma lipid levels in obese children.
The aim of this study was to examine the association between APOE genetic variants and the BMI and lipid profile in an Iranian cohort.
Samples were obtained from subjects who participated in a study based on the WHO-designed MONICA (multinational monitoring of trends and determinants in cardiovascular disease) study for coronary artery disease risk assessment in Zone 17 of Tehran. The study was approved by the local ethical committee. Informed consent was obtained from all subjects included in this study.
Subjects (n=320) were recruited. The level of triglyceride (TG) and total serum cholesterol was tested for all subjects in this study. Genotyping for APOE was carried using polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP)technique.
Levels of significance were determined using contingency tables by either Chi-square or Fisher exact analysis using the STATA (v8) software. The analysis of regression and significance of differences for level of cholesterol and TG was established by one-way analysis of variance followed by Dunnett post hoc multiple comparison tests using SPSS software Version 11.5.
The frequency of allele E2 was significantly higher in patients with total serum cholesterol level <200 mg/dl (P 0.01 OR 2.1 95% CI 1.1-4.2).
The association found in this study between allele E2 and lower total cholesterol level had been reported in previous studies. We have also observed that the frequency of genotype E2/E3 and E2/E4 was significantly higher in patients with normal total serum cholesterol level compared to patients with abnormal cholesterol (P=0.003 OR 2.4 95% CI; 1.3-4.6). Our data needs to be repeated in a larger population with more information for serum LDL and HDL levels and their subgroups.
载脂蛋白E（APOE）是人类血脂水平的主要调节因子。已报道了多种APOE基因等位变体，包括E2、E3和E4。最近的研究表明，APOE在肥胖以及肥胖儿童的体重指数（BMI）增加和血脂水平升高中发挥作用。
本研究旨在探讨伊朗人群中APOE基因变体与BMI和血脂谱之间的关联。
样本取自参与基于世界卫生组织设计的莫尼卡（MONICA，心血管疾病趋势和决定因素多国监测）研究的受试者，该研究用于德黑兰第17区的冠状动脉疾病风险评估。该研究得到了当地伦理委员会的批准。本研究纳入的所有受试者均获得了知情同意。
招募了320名受试者。对本研究中的所有受试者进行甘油三酯（TG）和总血清胆固醇水平检测。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对APOE进行基因分型。
使用STATA（v8）软件通过卡方检验或Fisher精确分析，利用列联表确定显著性水平。使用SPSS软件11.5版通过单因素方差分析，随后进行Dunnett事后多重比较检验，建立胆固醇和TG水平的回归分析及差异显著性分析。
总血清胆固醇水平<200mg/dl的患者中，等位基因E2的频率显著更高（P<0.01，OR 2.1，95%CI 1.1 - 4.2）。
本研究中发现的等位基因E2与较低总胆固醇水平之间的关联在先前的研究中已有报道。我们还观察到，总血清胆固醇水平正常的患者中，基因型E2/E3和E2/E4的频率显著高于胆固醇异常的患者（P = 0.003，OR 2.4，95%CI；1.3 - 4.6）。我们的数据需要在更大的人群中重复验证，并获取更多关于血清低密度脂蛋白和高密度脂蛋白水平及其亚组的信息。
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