Long Shi-yin, Zhang Xue-mei, Fu Ming-de, Xu Yan-hua, Liu Bing-wen
Department of Biochemistry, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Dec;21(6):615-8.
To investigate apolipoprotein E(apoE) polymorphism and its relationship with serum lipids and apolipoprotein, serum high density lipoprotein (HDL) subclasses in patients with hyperlipidemia(HL).
APOE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 112 patients with hyperlipidemia and 73 healthy subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.
APOE3/3 genotypes and allele epsilon3 frequency in HL group and control group were both the highest. In HL group, the genotype of APOE2 had higher serum APOE/CIII ratio and lower HDL3b levels, compared with the genotype of APOE3 (P<0.05). In control group, the genotype of apoE2 had higher serum triglycerides, APOE levels and APOE/CIII ratio, compared with the genotype of APOE3 and APOE4 (P<0.05).
Polymorphism of APOE gene may relate to the distribution of HDL particles.
探讨高脂血症（HL）患者载脂蛋白E（apoE）基因多态性及其与血脂、载脂蛋白、血清高密度脂蛋白（HDL）亚类的关系。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测APOE基因型。采用二维凝胶电泳结合免疫检测法测定112例高脂血症患者和73例健康对照者血清HDL亚类。
HL组和对照组中APOE3/3基因型及等位基因ε3频率均最高。在HL组中，与APOE3基因型相比，APOE2基因型患者血清APOE/CIII比值较高，HDL3b水平较低（P<0.05）。在对照组中，与APOE3和APOE4基因型相比，apoE2基因型患者血清甘油三酯、APOE水平及APOE/CIII比值较高（P<0.05）。
APOE基因多态性可能与HDL颗粒的分布有关。
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