载脂蛋白E基因多态性与冠心病早发

Apolipoprotein E polymorphism in the early onset of coronary heart disease.

作者信息

Yang Z, Zhu T, Ma G, Yin H, Qian W, Zhang F, Cao K, Ma W

机构信息

Department of Cardiology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.

出版信息

Chin Med J (Engl). 2001 Sep;114(9):983-5.

PMID:11780396

Abstract

OBJECTIVE

To assess the relationship between apolipoprotein E (apoE) polymorphism and the early onset of coronary heart disease (CHD) and the effect of apoE on lipids and lipoproteins in healthy Chinese subjects.

METHODS

Sixty-eight patients with CHD younger than 55 years (CHD1), 136 patients with CHD older than 65 years (CHD2), and 136 healthy subjects were enrolled, and their plasma levels of triglyceride (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were determined. The apoE genotypes were identified by polymerase chain reaction-restriction fragment length polymorphism.

RESULTS

apoE 3/4 genotype and E4 allele frequency in the CHD1 group were higher than those in the CHD2 group and healthy subjects, while no differences were found between CHD2 and healthy subjects. Meanwhile, the plasma levels of TC and low density lipoprotein cholesterol (LDL-C) were higher in the CHD2 group than in both CHD1 group and healthy subjects. Each apoE isoprotein has variable TC and LDL-C levels that is E2 (E2/2 + E2/3) < E3(E3/3) < E4(E4/4 + E3/4).

CONCLUSION

apoE is one of the genetic factors that affect TC and LDL-C levels, and apoE 4 has a very close relation to CHD, suggesting that apoE 4 is an independent genetic factor of the early onset of CHD.

摘要

目的

评估载脂蛋白E（apoE）基因多态性与冠心病（CHD）早发之间的关系，以及apoE对健康中国受试者血脂和脂蛋白的影响。

方法

纳入68例年龄小于55岁的冠心病患者（CHD1组）、136例年龄大于65岁的冠心病患者（CHD2组）和136例健康受试者，测定其血浆甘油三酯（TG）、总胆固醇（TC）和高密度脂蛋白胆固醇（HDL-C）水平。采用聚合酶链反应-限制性片段长度多态性方法鉴定apoE基因型。

结果

CHD1组apoE 3/4基因型和E4等位基因频率高于CHD2组和健康受试者，而CHD2组与健康受试者之间无差异。同时，CHD2组血浆TC和低密度脂蛋白胆固醇（LDL-C）水平高于CHD1组和健康受试者。各apoE同工蛋白的TC和LDL-C水平不同，即E2（E2/2 + E2/3）< E3（E3/3）< E4（E4/4 + E3/4）。