Radial oxygen gradients over rat cortex arterioles.

PURPOSE

We present the results of the visualisation of radial oxygen gradients in rats' cortices and their potential use in neurocritical management.

METHODS

PO₂ maps of the cortex of ten sedated, intubated and controlled ventilated Wistar rats were obtained with a camera (SensiMOD, PCO, Kelheim, Germany). Those pictures were analysed and edited by a custom-made software. A virtual matrix, designed to evaluate the cortical O₂ partial pressure, was placed vertically to the artery under investigation, and afterwards multiple regions of interest were measured (width 10 pixels, length 15-50 pixels). The results showed a map of the cerebral oxygenation, which allowed us to calculate radial oxygen gradients over arterioles. Three groups were defined according to the level of the arterial pO₂: PaO₂ < 80, PaO₂ 80-120 and PaO₂ > 120. Gradients were analysed from the middle of the vessel to its border (1), from the border into the parenchyma next to the vessel (2) and a combination of both (3).

RESULTS

Gradient 1 showed significantly different cortical pO₂ values between the three different groups. The mean pO₂ values were 2.62, 5.29 and 5.82 mmHg/mm. Gradient 2 measured 0.56, 0.90 and 1.02 mmHg/mm respectively. Gradient 3 showed significant results between the groups with values of 3.18, 6.19 and 6.84 mmHg/mm.

CONCLUSION

Using these gradients, it is possible to describe and compare the distribution of oxygen to the brain parenchyma. With the presented technique, it is possible to detect pO₂ changes in the oxygen supply of the brain cortex.