The Genomics Research Center, Academia Sinica, Taipei, Taiwan, Republic of China.
Rapid Commun Mass Spectrom. 2010 Sep 15;24(17):2569-74. doi: 10.1002/rcm.4677.
To date, mass spectrometric analysis of biomolecules has been primarily performed with either matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization (ESI). In this work, ultrasound produced by a simple piezoelectric device is shown as an alternative method for soft ionization of biomolecules. Precursor ions of proteins, saccharides and fatty acids showed little fragmentation. Cavitation is considered as a primary mechanism for the ionization of biomolecules.
迄今为止，生物分子的质谱分析主要采用基质辅助激光解吸/电离（MALDI）或电喷雾电离（ESI）来进行。在这项工作中，我们展示了一种简单的压电装置产生的超声波可作为生物分子软电离的替代方法。蛋白质、糖和脂肪酸的前体离子几乎没有碎片化。空化作用被认为是生物分子电离的主要机制。
