National Veterinary Institute, P.O. Box 750 Sentrum, N-0106 Oslo, Norway.
Rapid Commun Mass Spectrom. 2010 Sep 15;24(17):2641-9. doi: 10.1002/rcm.4687.
This paper reports the mass spectra, obtained after electron ionisation (EI) at 70 eV, of 34 trichothecenes and five culmorin compounds after acylation with pentafluoropropionic anhydride. The derivatised fungal metabolites were separated by gas chromatography, and the mass spectra were obtained by scanning of a single quadrupole mass filter in the scan range m/z 200-900. The fragmentation pathways of three trichothecenes (triacetyl-deoxynivalenol, 4,15-diacetoxy-scirpenol, T-2 toxin) have been studied in more detail by linked scan-high-resolution mass spectrometry. The most common trichothecenes are today more often routinely analysed using LC/MS-based methodologies. However, EI-MS may give complementary structural information, and the data that are summarised in this article may help to identify analogues of one of the largest class of mycotoxins, the tricothecenes, as well as culmorin compounds that are commonly co-produced by Fusarium culmorum and F. graminearum in cultures or naturally contaminated samples.
本文报道了 34 种单端孢霉烯族化合物和 5 种杯曲霉素化合物经五氟丙酸酐酰化后，在 70eV 电子电离（EI）下获得的质谱。衍生化的真菌代谢物通过气相色谱分离，并通过在扫描范围 m/z 200-900 中扫描单个四极杆质量滤波器获得质谱。通过链接扫描-高分辨率质谱对三种单端孢霉烯族化合物（脱乙酰基脱氧雪腐镰刀菌烯醇、4,15-二乙酰氧基雪腐镰刀菌烯醇、T-2 毒素）的碎裂途径进行了更详细的研究。目前，基于 LC/MS 的方法更常用于常规分析常见的单端孢霉烯族化合物。然而，EI-MS 可能会提供补充的结构信息，本文总结的数据可能有助于鉴定最大类霉菌毒素之一——单端孢霉烯族化合物的类似物，以及在培养物或自然污染样品中由禾谷镰刀菌和禾谷镰刀菌共同产生的杯曲霉素化合物。
