Department of Molecular Science and Technology, Ajou University, Suwon 443-759, Korea.
J Tissue Eng Regen Med. 2011 May;5(5):410-4. doi: 10.1002/term.328. Epub 2010 Aug 26.
Several recent studies have demonstrated that stem cell differentiation can be generated by derivatives of retinoic acid. In this study we chose retinoic acid (RA) for inducing neural differentiation of rat muscle-derived stem cells (rMDSCs). rMDSCs were pre-induced with 10 ng/ml basic fibroblast growth factor (bFGF) and then treated with 2 μM RA. After stimulation, RA induced rMDSCs to have a neural-like morphology after 1-7 days of in vitro differentiation. In the results of immunocytochemistry, rMDSC treated with RA showed abundant positive cells against the neuronal markers neuronal-specific enolase (NSE) and tubulin-βIII (Tuj1). Also, 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)-positive cells were observed, indicating oligodendrocyte lineage cells. However, positive cells against glial fibrillary acidic protein (GFAP), marker of astrocytes, were not detected. The mRNA profile of these cells included higher expression of NSE compared with those of non-treated cells in real-time PCR. From the data in this work, we suggest that rMDSCs can trans-differentiate into a neural-like phenotype under the RA conditions.
几项最近的研究表明，干细胞分化可以通过维甲酸的衍生物产生。在这项研究中，我们选择维甲酸（RA）诱导大鼠肌肉源性干细胞（rMDSCs）的神经分化。rMDSCs 先用 10ng/ml 碱性成纤维细胞生长因子（bFGF）预诱导，然后用 2μM RA 处理。刺激后，RA 在体外分化 1-7 天后诱导 rMDSCs 具有神经样形态。免疫细胞化学结果显示，用 RA 处理的 rMDSC 对神经元标志物神经元特异性烯醇化酶（NSE）和微管蛋白-βIII（Tuj1）呈丰富的阳性细胞。此外，还观察到 2'，3'-环核苷酸 3'-磷酸二酯酶（CNPase）阳性细胞，表明少突胶质细胞谱系细胞。然而，没有检测到胶质纤维酸性蛋白（GFAP）阳性细胞，GFAP 是星形胶质细胞的标志物。这些细胞的 mRNA 谱在实时 PCR 中包括与未处理细胞相比 NSE 的更高表达。从这项工作的数据来看，我们认为 rMDSC 在 RA 条件下可以转分化为神经样表型。
