Raju K S, Cartwright A, Hirsch F W, Wu B C, Nall K N, Morris H P, Busch H
Cancer Res. 1978 Jul;38(7):1922-8.
Protein 64/7.2 (molecular weight/isoelectric point) is present in the cytosol of several hepatomas including the Novikoff hepatoma and Morris hepatomas 9618A, 7794A, and 3924A, but it is not present in liver or 18-hr regenerating liver. Quantitatively, its concentration was highest in Novikoff hepatoma (150 microgram/g tissue) and Morris hepatoma 3924A (550 microgram/g tissue), which are rapid-growing tumors, less in Morris hepatoma 7794A (72 microgram/g tissue), which is of intermediate growth rate, and least in the slow-growing Morris hepatoma 9618A (25 microgram/g tissue). Protein 64/7.2 was isolated from Novikoff hepatoma ascites cells in high purity as shown by its migration as a single band on one-dimensional acid-urea-polyacrylamide gels and a single spot on two-dimensional isoelectric focusing sodium dodecyl sulfate-polyacrylamide gels. Its amino acid composition has an acidic to basic amino acid ratio of 1.6. Its amino-terminal amino acid is lysine, and its carboxyl-terminal amino acid is glycine. Interestingly, the amino acid composition is strikingly similar to that of the phosphorylated Novikoff hepatoma chromatin Protein Cg', partially characterized in our laboratory.
蛋白质64/7.2（分子量/等电点）存在于包括诺维科夫肝癌以及莫里斯肝癌9618A、7794A和3924A在内的几种肝癌细胞溶质中，但在正常肝脏或18小时再生肝脏中不存在。从数量上看，其浓度在快速生长的诺维科夫肝癌（150微克/克组织）和莫里斯肝癌3924A（550微克/克组织）中最高，在生长速度中等的莫里斯肝癌7794A（72微克/克组织）中较少，而在生长缓慢的莫里斯肝癌9618A（25微克/克组织）中最少。蛋白质64/7.2从诺维科夫肝癌腹水细胞中以高纯度分离出来，这在一维酸-尿素-聚丙烯酰胺凝胶上表现为单一条带迁移，在二维等电聚焦十二烷基硫酸钠-聚丙烯酰胺凝胶上表现为单个斑点。其氨基酸组成的酸性氨基酸与碱性氨基酸比例为1.6。其氨基末端氨基酸是赖氨酸，羧基末端氨基酸是甘氨酸。有趣的是，其氨基酸组成与我们实验室部分鉴定的磷酸化诺维科夫肝癌染色质蛋白Cg'惊人地相似。
