Raju K S, Morris H P, Busch H
Cancer Res. 1980 May;40(5):1623-9.
Protein 45/7.8 (molecular weight X 10(-3)/isoelectric point) was found in the cytosol of several rapidly growing hepatomas including Morris hepatomas 3924A and 9618A2 and Novikoff hepatoma. It was not found in Morris hepatomas 7794A and 8999, which have intermediate growth rates, or in the slow-growing Morris hepatoma 9618A, normal rat liver, 18-hr regenerating rat liver, or livers of fetal rats. Protein 45/7.8 was isolated under nondenaturing conditions from Morris hepatomas 3924A and 9618A2 and Novikoff hepatoma in high purity by purification in three steps: ammonium sulfate fractionation; diethylaminoethyl cellulose chromatography; and hydroxylapatite chromatography. The protein was highly purified as shown by two-dimensional, isoelectric-focusing sodium dodecyl sulfate:polyacrylamide gels. The amino acid composition of protein 45/7.8 from the three tumors studied was very similar; the acid of protein 45/7.8 was proline, and its COOH terminus amino acid was tyrosine. Protein 43/8.1 found in normal liver is different from hepatoma protein 45/7.8 by amino terminus, amino acid composition, and tryptic peptides.
在几种快速生长的肝癌细胞溶质中发现了蛋白质45/7.8（分子量×10(-3)/等电点），包括莫里斯肝癌3924A和9618A2以及诺维科夫肝癌。在生长速度中等的莫里斯肝癌7794A和8999、生长缓慢的莫里斯肝癌9618A、正常大鼠肝脏、18小时再生大鼠肝脏或胎鼠肝脏中未发现该蛋白质。通过三步纯化，即硫酸铵分级分离、二乙氨基乙基纤维素层析和羟基磷灰石层析，在非变性条件下从莫里斯肝癌3924A和9618A2以及诺维科夫肝癌中高纯度分离出蛋白质45/7.8。如二维等电聚焦十二烷基硫酸钠：聚丙烯酰胺凝胶所示，该蛋白质得到了高度纯化。所研究的三种肿瘤中蛋白质45/7.8的氨基酸组成非常相似；蛋白质45/7.8的酸性氨基酸是脯氨酸，其COOH末端氨基酸是酪氨酸。正常肝脏中发现的蛋白质43/8.1在氨基末端、氨基酸组成和胰蛋白酶肽方面与肝癌蛋白质45/7.8不同。
