Ward J M, Vlahakis G
J Natl Cancer Inst. 1978 Sep;61(3):807-11.
Thirty C3H-AvyfB male mice from 3 to 15 months of age were killed, and the genesis of their hepatocellular neoplasms was studied. As the mice aged, the number of tumors per liver and mean diameter of the tumors increased. Histologically, the smallest tumors were composed of solid sheets of cells and were better differentiated than the largest tumors. Small foci of hepatocytes in prominent trabeculae formation was a common characteristic of large tumors. In addition, 18 metastatic hepatocellular carcinomas observed in aged C3H-AvyfB, C3HfC, C3H, C3HfB, (C3HfB x YBR)F1, or C57BL/6 mice were also included for a morphologic study of their primary and metastatic lesions. Invariably, the pulmonary metastases were composed of a well-differentiated sheet of hepatocytes that resembled those in the solid liver tumors. The smallest hepatocellular neoplasms appear to represent early stages in the development of metastatic hepatocellular carcinoma.
处死30只3至15月龄的C3H-AvyfB雄性小鼠，并研究其肝细胞肿瘤的发生情况。随着小鼠年龄增长，每只肝脏的肿瘤数量和肿瘤平均直径均增加。组织学上，最小的肿瘤由实性细胞片组成，比最大的肿瘤分化更好。大肿瘤的一个共同特征是在突出的小梁形成中有小的肝细胞灶。此外，还纳入了在老年C3H-AvyfB、C3HfC、C3H、C3HfB、(C3HfB×YBR)F1或C57BL/6小鼠中观察到的18例转移性肝细胞癌，对其原发灶和转移灶进行形态学研究。肺转移灶总是由一层分化良好的肝细胞组成，类似于实性肝肿瘤中的肝细胞。最小的肝细胞肿瘤似乎代表了转移性肝细胞癌发展的早期阶段。
