Kill I R, Shall S
Laboratory of Cell and Molecular Biology, School of Biological Sciences, University of Sussex, Brighton, England.
J Cell Sci. 1990 Nov;97 ( Pt 3):473-8. doi: 10.1242/jcs.97.3.473.
The characteristic limited reproductive life-span of normal human fibroblasts in culture is due to a steadily decreasing fraction of cells able to proliferate in the standard rich growth media. We have observed that restricting the growth factor supply to old cells for variable lengths of time in culture increases the fraction of cells that can enter S-phase; although these cells do not go on to divide. Thus, it seems that there is a transient phase between the proliferating state and the irreversibly post-mitotic, senescent state. Perhaps a 'quiescent-G0' state, which can be maintained in the presence of growth factors, is a stage on the pathway to mortalization and senescence.
培养的正常人成纤维细胞具有有限的特征性繁殖寿命，这是由于在标准的丰富生长培养基中能够增殖的细胞比例稳步下降。我们观察到，在培养过程中，对老龄细胞限制不同时长的生长因子供应，可增加能够进入S期的细胞比例；尽管这些细胞不会继续分裂。因此，似乎在增殖状态和不可逆的有丝分裂后衰老状态之间存在一个过渡阶段。也许可以在生长因子存在的情况下维持的“静止-G0”状态，是走向死亡和衰老途径上的一个阶段。
