Tyndall D A, Cook C S
Department of Diagnostic Sciences, University of North Carolina, Chapel Hill 27599-7450.
J Craniofac Genet Dev Biol. 1990;10(4):353-61.
An investigation was undertaken to evaluate ocular size and shape by objective and subjective criteria to establish the incidence and laterality of spontaneous microphthalmia in the C57B1/6J mouse strain. The purpose was to provide useful information of normal and abnormal ocular development in a laboratory strain frequently used for teratogenic studies. Ten-week-old C57B1/6J mice were time mated and sacrificed at gestational day 14. Eye normality was evaluated subjectively using veterinary ophthalmology methods, and objectively by a computer derived method. A distinct laterality was noted in the right eye when compared to the left eye. Degree of laterality varied with assessment methodology (19.0% vs. 6.9% and 8.9% vs. 4.0% for the subjective and objective methods respectively). Mean eye area and histogram distributions for the right eye were consistently smaller than the left eye for all anatomical rating groups, thereby suggesting that asymmetry found in the C57B1/6J may be due to an inherent discrepancy in eye size in the strain. The data from this study suggest that when C57B1/6J mouse eyes are used as end points for teratological testing each eye should be treated as an independent unit of measure.
开展了一项调查，通过客观和主观标准评估眼部大小和形状，以确定C57B1/6J小鼠品系中自发性小眼畸形的发生率和单侧性。目的是为常用于致畸研究的实验室品系中正常和异常眼部发育提供有用信息。对10周龄的C57B1/6J小鼠进行定时交配，并在妊娠第14天处死。使用兽医眼科方法主观评估眼部正常情况，并通过计算机衍生方法进行客观评估。与左眼相比，右眼存在明显的单侧性。单侧性程度因评估方法而异（主观方法分别为19.0%对6.9%，客观方法分别为8.9%对4.0%）。在所有解剖评级组中，右眼的平均眼面积和直方图分布始终小于左眼，因此表明C57B1/6J中发现的不对称可能是由于该品系眼部大小存在固有差异。这项研究的数据表明，当将C57B1/6J小鼠的眼睛用作致畸测试的终点时，每只眼睛都应被视为一个独立的测量单位。
