Lin C W, Kirley S D, Khaw A H, Zhang D S, Prout G R
Urology Research Laboratory, Massachusetts General Hospital, Boston 02114.
J Occup Med. 1990 Sep;32(9):910-6. doi: 10.1097/00043764-199009000-00031.
Monoclonal antibodies (Mabs) to human tumor antigens have potential for tumor detection and treatment. For bladder carcinoma, the detection of exfoliated tumor cells in urinary specimens may be accomplished with Mabs reacting to tumor cell-surface components. This method may be useful for screening and monitoring carcinogen-exposed workers. A Mab generated by our laboratory, 3G2-C6, reacts with high affinity to a cell-surface component expressed by bladder tumor cells. The potential utility of this Mab in detecting exfoliated tumor cells was evaluated in bladder wash specimens. The Mab method detected positive cells in 87% (56/64) of specimens from patients with bladder cancer, including a great majority with grade 1 tumor and carcinoma in situ, superior to the routine cytology done on the same specimens. Cells in specimens from patients with urinary calculi, chronic cystitis, and history of bladder cancer also reacted with the Mab, suggesting that other stimuli can induce antigen expression. The Mab method can also be performed on urine samples, thus allowing evaluation of the ability of the Mab to identify premalignant, malignant, and other abnormal exfoliated cells in urine. The Mab method represents a unique opportunity to develop noninvasive detection of bladder cancer and to monitor and screen bladder cancer high-risk groups.
针对人类肿瘤抗原的单克隆抗体（Mabs）在肿瘤检测和治疗方面具有潜力。对于膀胱癌，利用与肿瘤细胞表面成分发生反应的单克隆抗体，可实现对尿液标本中脱落肿瘤细胞的检测。该方法可能有助于筛查和监测接触致癌物的工人。我们实验室制备的一种单克隆抗体3G2-C6，能与膀胱肿瘤细胞表达的一种细胞表面成分发生高亲和力反应。在膀胱冲洗标本中评估了这种单克隆抗体在检测脱落肿瘤细胞方面的潜在效用。单克隆抗体法在87%（56/64）的膀胱癌患者标本中检测到阳性细胞，其中包括大多数1级肿瘤和原位癌患者，优于对相同标本进行的常规细胞学检查。尿路结石、慢性膀胱炎患者以及有膀胱癌病史患者的标本中的细胞也与单克隆抗体发生反应，这表明其他刺激因素可诱导抗原表达。单克隆抗体法也可对尿液样本进行检测，从而能够评估单克隆抗体识别尿液中癌前、恶性及其他异常脱落细胞的能力。单克隆抗体法为开发膀胱癌的无创检测以及监测和筛查膀胱癌高危人群提供了独特契机。
