Huland E, Huland H, Meier T, Baricordi O, Fradet Y, Grossman H B, Hodges G M, Messing E M, Schmitz-Draeger B J
Klinikum Steglitz, Free University of Berlin, Germany.
J Urol. 1991 Dec;146(6):1631-6. doi: 10.1016/s0022-5347(17)38202-2.
Quantitative urinary immunocytology with our monoclonal antibody (mab) 486p 3/12 proved to be valuable for diagnostic use in bladder-cancer patients' urine, especially in the followup of patients with superficial bladder carcinoma. To evaluate the use of other monoclonal antibodies in bladder cancer, we compared 15 mabs directed against bladder-tumor-associated antigens from seven research groups in a broad panel of cellular and tissue specimens (bladder tumor, prostatic adenoma, and kidney stone). Quantitative evaluation was done in cytocentrifuged preparations and tissue specimens. None of the 15 mabs was bladder-tumor-specific. All 15 stained normal urothelium to some extent and six stained granulocytes. Each of the 15 seemed to identify a different cellular antigen, as can be clearly demonstrated by the staining pattern of different regions in the normal kidney. The sensitivity of quantitative urinary immunocytology in bladder-tumor patients can be improved by using a panel, rather than one mab in bladder-tumor patients, but specificity decreases simultaneously. A main reason for the poor specificity of quantitative urinary immunocytology with all 15 mabs is that false-positive results are obtained with all mabs in kidney-stone patients. Our quantitative urinary immunocytology method is a general tool for the diagnostic use of all mabs in bladder-tumor patients. Mabs that have a high sensitivity might be useful in the followup of patients with superficial bladder carcinoma. None of the 15 mabs (because of their poor specificity) seems to be helpful in quantitative urinary immunocytology for screening a population for bladder carcinoma.
用我们的单克隆抗体（mab）486p 3/12进行的定量尿免疫细胞学检查被证明对膀胱癌患者尿液的诊断有价值，特别是在浅表性膀胱癌患者的随访中。为了评估其他单克隆抗体在膀胱癌中的应用，我们在广泛的细胞和组织标本（膀胱肿瘤、前列腺腺瘤和肾结石）中比较了来自七个研究小组的15种针对膀胱肿瘤相关抗原的单克隆抗体。在细胞离心涂片和组织标本中进行定量评估。这15种单克隆抗体均非膀胱肿瘤特异性。所有15种抗体均在一定程度上对正常尿路上皮染色，6种对粒细胞染色。这15种抗体中的每一种似乎都识别一种不同的细胞抗原，这可以通过正常肾脏不同区域的染色模式清楚地证明。在膀胱癌患者中，使用一组单克隆抗体而不是一种单克隆抗体可以提高定量尿免疫细胞学的敏感性，但特异性会同时降低。使用所有15种单克隆抗体进行定量尿免疫细胞学检查特异性差的一个主要原因是肾结石患者使用所有单克隆抗体都会获得假阳性结果。我们的定量尿免疫细胞学方法是在膀胱癌患者中诊断使用所有单克隆抗体的通用工具。具有高敏感性的单克隆抗体可能对浅表性膀胱癌患者的随访有用。这15种单克隆抗体（因其特异性差）似乎都无助于通过定量尿免疫细胞学对人群进行膀胱癌筛查。
