Finley P R, Schifman R B, Williams R J, Lichti D A
Clin Chem. 1978 Jun;24(6):931-3.
We describe a method for measuring high-density lipoprotein cholesterol. MgCl2 and dextran sulfate are used to precipitate all low-density and very-low-density lipoproteins. The supernate contains only high-density lipoproteins, the cholesterol concentration of which is estimated by an enzymic method, with a discrete analyzer (Abbott Bichromatic Analyzer). Concentration and instrument response are linearly related to 50 mg/liter. The precision of the method is excellent in the range of clinical interest (100 to 1000 mg of cholesterol per liter). The precision and efficiency of the precipitation are shown at various concentrations of high-density lipoprotein cholesterol. The method was compared to that of two laboratories in the Cooperative Lipoprotein Phenotyping Study group by testing a number of split samples, and agreement was good.
我们描述了一种测量高密度脂蛋白胆固醇的方法。氯化镁和硫酸葡聚糖用于沉淀所有低密度和极低密度脂蛋白。上清液仅含有高密度脂蛋白，其胆固醇浓度通过酶法用离散分析仪（雅培双色分析仪）进行估算。浓度与仪器响应在50毫克/升范围内呈线性相关。该方法在临床相关范围内（每升100至1000毫克胆固醇）的精密度极佳。在不同浓度的高密度脂蛋白胆固醇条件下展示了沉淀的精密度和效率。通过检测多个分割样本，将该方法与合作脂蛋白表型研究组中两个实验室的方法进行了比较，结果一致性良好。
