Warnick G R, Mayfield C, Benderson J, Chen J S, Albers J J
Am J Clin Pathol. 1982 Nov;78(5):718-23. doi: 10.1093/ajcp/78.5.718.
Two methods using commercial kits for high density lipoprotein (HDL) cholesterol quantitation were compared with the Lipid Research Clinics (LRC) procedures. HDL cholesterol quantitations on 50 patient specimens by the Lancer HDL cholesterol Rapid Stat Kit (Lancer) with phosphotungstate-Mg2+ precipitation and enzymic cholesterol assay averaged 424 mg/L, and by a method with dextran sulfate-Mn2+-polyethylene glycol (dextran sulfate) precipitation and enzymic cholesterol assay averaged 474 mg/L. By comparison, the LRC method (heparin-Mn2+ precipitation combined with a Liebermann-Burchard reagent cholesterol assay) averaged 478 mg/L. Supernates obtained by the three precipitation methods had similar cholesterol values when analyzed by the LRC assay, suggesting that the observed differences were primarily due to differences between the cholesterol assays. Results were consistent with underestimation by the enzymic assay of cholesterol in the supernates, offset by a positive interference of Mn2+ in the dextran sulfate-produced supernates. Among-day CVs of 4-5% were observed for the Lancer method, and 6-7% for the dextran sulfate method. Sedimentation of precipitates in hypertriglyceridemic specimens was excellent by both methods.
将两种使用商业试剂盒定量高密度脂蛋白（HDL）胆固醇的方法与脂质研究临床中心（LRC）的方法进行了比较。使用磷钨酸 - Mg2 + 沉淀法和酶法胆固醇测定的Lancer HDL胆固醇快速检测试剂盒（Lancer）对50份患者标本进行HDL胆固醇定量，平均值为424mg/L；使用硫酸葡聚糖 - Mn2 + - 聚乙二醇（硫酸葡聚糖）沉淀法和酶法胆固醇测定的方法，平均值为474mg/L。相比之下，LRC方法（肝素 - Mn2 + 沉淀法结合利伯曼 - 布查德试剂胆固醇测定法）平均值为478mg/L。当通过LRC测定法分析时，三种沉淀方法获得的上清液具有相似的胆固醇值，这表明观察到的差异主要是由于胆固醇测定方法之间的差异。结果与酶法测定上清液中胆固醇时的低估一致，硫酸葡聚糖产生的上清液中Mn2 + 的正干扰抵消了这种低估。Lancer方法的日间变异系数为4 - 5%，硫酸葡聚糖方法为6 - 7%。两种方法对高甘油三酯血症标本中沉淀物的沉淀效果都很好。
