Lenzi R, Alpini G, Liu M H, Rand J H, Tavoloni N
Department of Medicine, Polly Annenberg Levee Hematology Center, Mount Sinai School of Medicine, City University of New York, N.Y.
Liver. 1990 Dec;10(6):372-9. doi: 10.1111/j.1600-0676.1990.tb00483.x.
To determine whether von Willebrand Factor (vWF) is a valid marker of liver endothelial cells, we determined vWF immunoreactivity in rat and guinea pig liver sections and in smears of elutriated nonparenchymal cells isolated from these two species. In frozen sections, positive staining for vWF was seen only in the endothelium lining large hepatic vessels in both species, and no immunoactivity was detected in the sinusoids. On the other hand, immunohistochemical staining for vimentin (a marker of mesenchymal cells) showed positive reaction throughout the vascular and sinusoidal endothelial cells in both the rat and guinea pig liver. In fractions of elutriated rat and guinea pig nonparenchymal liver cells, which included almost exclusively liver endothelial cells, only 25-40% of the cells displayed a positive reaction for vWF. However, when these same fractions were stained for vimentin, 70-95% of the cells exhibited immunoreactivity. Most of the vWF-negative cells were not red and white blood cells, biliary epithelial and Kupffer cells, and hepatocytes, and had ultrastructural features of sinusoidal endothelial cells. We conclude that in both the rat and guinea pig, liver sinusoidal endothelial cells do not exhibit vWF immunoreactivity. Thus, in routine immunohistochemical assays, vWF is not an accurate marker of rat and guinea pig liver endothelial cells. Vimentin is more appropriate for this purpose, provided that other mesenchymal cells are separated or independently identified.
为了确定血管性血友病因子（vWF）是否是肝内皮细胞的有效标志物，我们检测了大鼠和豚鼠肝脏切片以及从这两个物种分离的淘洗非实质细胞涂片的vWF免疫反应性。在冰冻切片中，仅在两个物种的大型肝血管内衬的内皮细胞中观察到vWF阳性染色，而在肝血窦中未检测到免疫活性。另一方面，波形蛋白（间充质细胞标志物）的免疫组织化学染色显示大鼠和豚鼠肝脏的血管和血窦内皮细胞均呈阳性反应。在几乎仅包含肝内皮细胞的大鼠和豚鼠非实质肝细胞分馏物中，只有25%-40%的细胞对vWF呈阳性反应。然而，当对这些相同的分馏物进行波形蛋白染色时，70%-95%的细胞表现出免疫反应性。大多数vWF阴性细胞不是红细胞、白细胞、胆管上皮细胞、库普弗细胞和肝细胞，并且具有肝血窦内皮细胞的超微结构特征。我们得出结论，在大鼠和豚鼠中，肝血窦内皮细胞均不表现出vWF免疫反应性。因此，在常规免疫组织化学检测中，vWF不是大鼠和豚鼠肝内皮细胞的准确标志物。如果其他间充质细胞被分离或独立鉴定，波形蛋白更适合用于此目的。
